MF2SQN OGMP SEQUENCE UTILITIES MF2SQN NAME mf2sqn - Converts an OGMP masterfile into an SQN record that can be converted by NCBI's SEQUIN tool to the ASN.1 format, which in turn is suitable for submission to Genbank. SYNOPSIS mf2sqn [-d] [-t] [-g] -a author -m masterfile The files masterfile.sqn masterfile.gbf are created in the working directory Further, unless present, a masterfile.subInfo file will be created as well that contains general information about the submitter and the sequence. This file can be edited as pleases and the mf2sqn run a second time. DESCRIPTION This program converts a masterfile into an SQN file. The masterfile must absolutely be flawless; using the cleanmf(1) program is a good start, but mf2sqn is even more strict and may report errors that even cleanmf did not check. mf2sqn works in five stages: 1) The masterfile is read and a preliminary parsing of the annotations is done. Any error causes the program to report it and quit. 2) NCBI's validator is called, which may report more errors (in a very cryptic manner) but will not prevent the creation of the SQN dump. SWITCHES and OPTIONS -a author : All submitter file need to be in "/share/supported/apps/mf2sqn/" and have following format name "SubmitterName.sbt" (need to respect case). A template can be found in "SubmitterGeneric.sbt" (never remove it, it is used by mfannot online). Each file contain a text ASN.1 Submit-block object. The Submit-block contains contact information (to whom questions on the submission can be addressed) and a submission citation (which lists the authors who get scientific credit for the sequencing). -m masterfile : Is the masterfile for submission. -d : Create GenBank file in output. -t : Create Table file in output. -g : 'personal' gene_names_file; otherwise, the default gene_names.lst is used FILES masterfile.subinfo All information supplied by the user (such as submission information, references etc) are cached in this file, /share/supported/apps/ogmp/lib/ogmp_qualifiers.lst List of qualifiers /share/supported/apps/ogmp/lib/genome_name.lst Genome information database /share/supported/apps/ogmp/lib/gene_names.lst Gene information database /share/supported/apps/mf2sqn/SubmitterName.sbt $NCBI/data/gc.prt NCBI genetic codes database AUTHOR Pierre Rioux, Natacha Beck 2009-2011.