OGMP- DNA Sequencing strategy

DNA sequencing is performed by the dideoxy chain termination method (Sanger et al, 1977). Cycle-sequencing reactions with plasmid DNA are performed for Li-Cor sequencing, while standard reactions with T7 DNApol, 35S-dATP and single-stranded DNA templates are performed for clone "fingerprinting", which consists of only an A-reaction, instead of GATC-reactions.

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