Materials and methods
Axenic culture (UTEX #1298, ATCC #30004)
Culture and media
Cultured by Annette Coleman in a medium containing glucose,
yeast extract tryptone and soil water (A. Coleman et al
(1991), J. Protozool.38,129-135)
Purification of mtDNA
Isolation of total cellular DNA and subsequent separation of the
A+T rich fraction through CsCl/bisbenzimide centrifugation.
Performed by: A. Coleman.
Random fragments, generated by nebulization,
and size-fractionated (0.5-1.0 kbp; 1.0-3.0 kbp) cloned in Bluescript KS+.
Performed by: I. Plante (Unit)
Manual Sanger sequencing of single-stranded DNA, using
S label. 1 custom primer has been used for primer walking.
5% of readings were obtained with the LiCor automatic sequencer.
The assembly was done by Staden's bap.
Performed by: Y. Zhu (Unit).
mtDNAs (FMGP)* | Other
complete mtDNAs | Gene
names | Mitochondrial Genomics ]