Score E
Sequences producing significant alignments: (bits) Value
sp|O67043|QUEA_AQUAE S-ADENOSYLMETHIONINE:TRNA RIBOSYLTRANS... 587 e-167
gb|AAD35659.1|AE001732_4 (AE001732) S-adenosylmethionine tR... 293 1e-78
sp|P21516|QUEA_ECOLI S-ADENOSYLMETHIONINE:TRNA RIBOSYLTRANS... 266 1e-70
sp|P44595|QUEA_HAEIN S-ADENOSYLMETHIONINE:TRNA RIBOSYLTRANS... 259 2e-68
sp|O32054|QUEA_BACSU S-ADENOSYLMETHIONINE:TRNA RIBOSYLTRANS... 252 2e-66
gb|AAD53918.1|AF179611_2 (AF179611) S-adenosylmethionine tR... 239 2e-62
sp|Q55850|QUEA_SYNY3 S-ADENOSYLMETHIONINE:TRNA RIBOSYLTRANS... 230 1e-59
gi|4154891 (AE001471) S-ADENOSYLMETHIONINE TRNA RIBOSYLTRAN... 220 2e-56
sp|O25702|QUEA_HELPY S-ADENOSYLMETHIONINE:TRNA RIBOSYLTRANS... 219 3e-56
emb|CAA14676| (AJ235270) S-ADENOSYLMETHIONINE:tRNA RIBOSYLT... 190 2e-47
gb|AAF11138.1|AE002001_4 (AE002001) s-adenosylmethionine:tR... 182 3e-45
emb|CAA13168| (AJ231126) z63f [Vibrio cholerae] 115 4e-25
sp|O51053|QUEA_BORBU PUTATIVE S-ADENOSYLMETHIONINE:TRNA RIB... 104 8e-22
emb|CAA70098.1| (Y08885) S-adenosylmethionine: tRNA ribosyl... 60 3e-08
sp|O25549|RUVA_HELPY HOLLIDAY JUNCTION DNA HELICASE RUVA >g... 36 0.48
emb|CAA15008| (AJ235272) PROBABLE 3-HYDROXYACYL-COA DEHYDRO... 35 0.82
sp|Q9ZKW9|RUVA_HELPJ HOLLIDAY JUNCTION DNA HELICASE RUVA >g... 32 5.4
sp|O67043|QUEA_AQUAE S-ADENOSYLMETHIONINE:TRNA RIBOSYLTRANSFERASE-ISOMERASE (QUEUOSINE
BIOSYNTHESIS PROTEIN QUEA) >gi|2983418 (AE000712)
queuosine biosynthesis protein [Aquifex aeolicus]
Length = 334
Score = 587 bits (1496), Expect = e-167
Identities = 293/334 (87%), Positives = 293/334 (87%)
Query: 1 MRIEEFDYELPEELIAKYPAVPRHSARLMVLNRKDQSIKHDTFINLPDYLEEGDLLVFNN 60
MRIEEFDYELPEELIAKYPAVPRHSARLMVLNRKDQSIKHDTFINLPDYLEEGDLLVFNN
Sbjct: 1 MRIEEFDYELPEELIAKYPAVPRHSARLMVLNRKDQSIKHDTFINLPDYLEEGDLLVFNN 60
Query: 61 TKVIPARLYGRKPTGGRVEVVLTDFIKPDEWKALIGGKKIRPGLVIEVAPDFKVEVLEHI 120
TKVIPARLYGRKPTGGRVEVVLTDFIKPDEWKALIGGKKIRPGLVIEVAPDFKVEVLEHI
Sbjct: 61 TKVIPARLYGRKPTGGRVEVVLTDFIKPDEWKALIGGKKIRPGLVIEVAPDFKVEVLEHI 120
Query: 121 EEGKFRVKLLGEEPLKLIDKYGHIPIPPYLKREEEPIDRVYYQTIFAKEKGAVASPTASL 180
EEGKFRVKLLGEEPLKLIDKYGHIPIPPYLKREEEPIDRVYYQTIFAKEKGAVASPTASL
Sbjct: 121 EEGKFRVKLLGEEPLKLIDKYGHIPIPPYLKREEEPIDRVYYQTIFAKEKGAVASPTASL 180
Query: 181 HFSXXXXXXXXXXGINFAFITLHVSYGTFKPVKVERVEEHRVDPEYXXXXXXXXXXXXXX 240
HFS GINFAFITLHVSYGTFKPVKVERVEEHRVDPEY
Sbjct: 181 HFSEELLEKLKEKGINFAFITLHVSYGTFKPVKVERVEEHRVDPEYVKIPKETVEKIKET 240
Query: 241 XXXXXXXXXXXXXXXXXLETKPFEPFEGWTDLYIYPGFKFKVVDAMITNFHLPRSSLLIL 300
LETKPFEPFEGWTDLYIYPGFKFKVVDAMITNFHLPRSSLLIL
Sbjct: 241 KEKGKRVVAVGTTVVRALETKPFEPFEGWTDLYIYPGFKFKVVDAMITNFHLPRSSLLIL 300
Query: 301 VSAFAGREFILKAYREAVEKRYRFYSYGDGMLIL 334
VSAFAGREFILKAYREAVEKRYRFYSYGDGMLIL
Sbjct: 301 VSAFAGREFILKAYREAVEKRYRFYSYGDGMLIL 334
gb|AAD35659.1|AE001732_4 (AE001732) S-adenosylmethionine tRNA ribosyltransferase [Thermotoga
maritima]
Length = 335
Score = 293 bits (743), Expect = 1e-78
Identities = 155/339 (45%), Positives = 218/339 (63%), Gaps = 9/339 (2%)
Query: 1 MRIEEFDYELPEELIAKYPAVPRHSARLMVLNRKDQSIKHDTFINLPDYLEEGDLLVFNN 60
M++ EFDYELP ELIA+ P PR ++RLMVL+RK Q I+H F + +YLE GDLLV N
Sbjct: 1 MKVSEFDYELPPELIAQEPVEPRDASRLMVLHRKTQRIEHRIFREIIEYLEPGDLLVLNV 60
Query: 61 TKVIPARLYGRKPTGGRVEVVLTDFIKPDEWKALIG-GKKIRPGLVIEVAPDFKVEVLEH 119
+KVIPARLY RK TG +E++L + ++ WK L+ G+K++ G + + D L
Sbjct: 61 SKVIPARLYARKKTGASIEILLIERLEEGIWKCLVRPGQKVKKGTELVIDEDLSAVCLGR 120
Query: 120 IEEGKFRVKLLGEEPLKLIDKYGHIPIPPYLKREEEPIDRVYYQTIFAKEKGAVASPTAS 179
E+G +K ++ +LI + G P+PPY+K E P++R YQT++AKE+G+VA+PTA
Sbjct: 121 GEDGTRILKFQPQDD-RLIFEKGRTPLPPYIKNEV-PLER--YQTVYAKEEGSVAAPTAG 176
Query: 180 LHFSXXXXXXXXXXGINFAFITLHVSYGTFKPVKVERVEEHRVDPEYXXXXXXXXXXXXX 239
LHF+ G+ FA + LHV GTF+PVKVE VE+H++ E+
Sbjct: 177 LHFTPELIEKLKKKGVQFAEVVLHVGIGTFRPVKVEEVEKHKMHEEFYQVPKETVRKLRE 236
Query: 240 XXXXXXXXXXXXXXXXXXLET----KPFEPFEGWTDLYIYPGFKFKVVDAMITNFHLPRS 295
LET E + G TDL+IYP F+FK+VDA++TNFHLPRS
Sbjct: 237 TRERGNRIVAVGTTTVRTLETIARLPEQEEYVGKTDLFIYPPFEFKLVDALVTNFHLPRS 296
Query: 296 SLLILVSAFAGREFILKAYREAVEKRYRFYSYGDGMLIL 334
+LL+LV+AFAG++F+++AYREAV++RYRF+S+GD MLIL
Sbjct: 297 TLLMLVAAFAGKDFVMEAYREAVKRRYRFFSFGDAMLIL 335
sp|P21516|QUEA_ECOLI S-ADENOSYLMETHIONINE:TRNA RIBOSYLTRANSFERASE-ISOMERASE (QUEUOSINE
BIOSYNTHESIS PROTEIN QUEA) >gi|96353|pir||B38530
queuosine biosynthesis-related protein queA -
Escherichia coli >gi|147485 (M37702) queA [Escherichia
coli] >gi|1773089 (U82664) S-adenosylmethionine:tRNA
ribosyltransferase-isomerase [Escherichia coli]
>gi|1786606 (AE000147) synthesis of queuine in tRNA;
probably S-adenosylmethionine:tRNA
ribosyltransferase-isomerase [Escherichia coli]
Length = 356
Score = 266 bits (674), Expect = 1e-70
Identities = 141/343 (41%), Positives = 199/343 (57%), Gaps = 10/343 (2%)
Query: 1 MRIEEFDYELPEELIAKYPAVPRHSARLMVLNRKDQSIKHDTFINLPDYLEEGDLLVFNN 60
MR+ +F +ELPE LIA YP R S RL+ L+ ++ H TF +L D L GDLLVFNN
Sbjct: 1 MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFTDLLDKLNPGDLLVFNN 60
Query: 61 TKVIPARLYGRKPTGGRVEVVLTDFIKPDEWKALIGGKKI-RPGLVIEVAPDFKVEVLEH 119
T+VIPARL+GRK +GG++EV++ + A I K +PG + + D +
Sbjct: 61 TRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPKPGAELLLGDDESINATMT 120
Query: 120 IEEGK-FRVKLLGEEP-LKLIDKYGHIPIPPYLKREEEPIDRVYYQTIFAKEKGAVASPT 177
G F V+ E L +++ GH+P+PPY+ R +E DR YQT+++++ GAVA+PT
Sbjct: 121 ARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDADRELYQTVYSEKPGAVAAPT 180
Query: 178 ASLHFSXXXXXXXXXXGINFAFITLHVSYGTFKPVKVERVEEHRVDPEYXXXXXXXXXXX 237
A LHF G+ AF+TLHV GTF+PV+V+ +E+H + EY
Sbjct: 181 AGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQDVVDAV 240
Query: 238 XXXXXXXXXXXXXXXXXXXXLETKP-------FEPFEGWTDLYIYPGFKFKVVDAMITNF 290
LE+ EPF T ++IYPGF++KVVDA++TNF
Sbjct: 241 LAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPFFDDTQIFIYPGFQYKVVDALVTNF 300
Query: 291 HLPRSSLLILVSAFAGREFILKAYREAVEKRYRFYSYGDGMLI 333
HLP S+L++LVSAFAG + + AY+ AVE++YRF+SYGD M I
Sbjct: 301 HLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRFFSYGDAMFI 343
sp|P44595|QUEA_HAEIN S-ADENOSYLMETHIONINE:TRNA RIBOSYLTRANSFERASE-ISOMERASE (QUEUOSINE
BIOSYNTHESIS PROTEIN QUEA) >gi|1075199|pir||B64057
queuosine biosynthesis protein (queA) homolog -
Haemophilus influenzae (strain Rd KW20) >gi|1573210
(U32710) queuosine biosynthesis protein (queA)
[Haemophilus influenzae Rd]
Length = 363
Score = 259 bits (655), Expect = 2e-68
Identities = 138/353 (39%), Positives = 205/353 (57%), Gaps = 20/353 (5%)
Query: 1 MRIEEFDYELPEELIAKYPAVPRHSARLMVLNRKDQSIKHDTFINLPDYLEEGDLLVFNN 60
MR+ +F+++LP+ELIA+YP R S RL+ LN ++ I H TF ++ D ++EGDLL+FNN
Sbjct: 1 MRVSDFNFDLPDELIARYPKTDRVSCRLLQLNGENGEIFHRTFSDVLDLIDEGDLLIFNN 60
Query: 61 TKVIPARLYGRKPTGGRVEVVLTDFIKPDEWKALI-GGKKIRPGLVIEVAPDFKVE---- 115
T+VIPAR++GRK +GG++EV++ + + A I K + G + + D E
Sbjct: 61 TRVIPARMFGRKASGGKIEVLVERMLDEHRFLAHIRSSKSPKEGAELFLGEDKLGENNGI 120
Query: 116 --VLEHIEEGKFRVKLLGEEP--LKLIDKYGHIPIPPYLKREEEPIDRVYYQTIFAKEKG 171
V++ F V+L + L ++ GH+P+PPY+ R +E D+ YQT+++K G
Sbjct: 121 KAVMKARHGALFEVELSDKSTALLDVLQTIGHMPLPPYIDRPDEEADQECYQTVYSKVPG 180
Query: 172 AVASPTASLHFSXXXXXXXXXXGINFAFITLHVSYGTFKPVKVERVEEHRVDPEYXXXXX 231
AVA+PTA LHF G+NF F+TLHV GTF+PV+VE +E+H + EY
Sbjct: 181 AVAAPTAGLHFDENLLEKLKAKGVNFEFVTLHVGAGTFQPVRVENIEDHVMHAEYVEVSQ 240
Query: 232 XXXXXXXXXXXXXXXXXXXXXXXXXXLETKP-----------FEPFEGWTDLYIYPGFKF 280
+E+ EP+ T ++IYPG KF
Sbjct: 241 EVCNAIIATKKAGKRVIAVGTTSVRSIESAALSAEEFGNPDLIEPYFSDTSIFIYPGKKF 300
Query: 281 KVVDAMITNFHLPRSSLLILVSAFAGREFILKAYREAVEKRYRFYSYGDGMLI 333
+VVD +ITNFHLP S+L++LVSAFAG + + AY+ AV+++YRF+SYGD M I
Sbjct: 301 RVVDCLITNFHLPESTLIMLVSAFAGYKNTMNAYKHAVKEKYRFFSYGDAMFI 353
sp|O32054|QUEA_BACSU S-ADENOSYLMETHIONINE:TRNA RIBOSYLTRANSFERASE-ISOMERASE (QUEUOSINE
BIOSYNTHESIS PROTEIN QUEA) >gi|2635237|emb|CAB14732|
(Z99118) S-adenosylmethionine tRNA ribosyltransferase
[Bacillus subtilis]
Length = 342
Score = 252 bits (638), Expect = 2e-66
Identities = 134/344 (38%), Positives = 200/344 (57%), Gaps = 12/344 (3%)
Query: 1 MRIEEFDYELPEELIAKYPAVPRHSARLMVLNRKDQSIKHDTFINLPDYLEEGDLLVFNN 60
M+++ FD+ELPE LIA+ P R ++RLMVL++ + +F ++ + EGD LV NN
Sbjct: 1 MKVDLFDFELPERLIAQVPLEQRDASRLMVLDKHTGELTDSSFKHIISFFNEGDCLVLNN 60
Query: 61 TKVIPARLYGRKP-TGGRVEVVLTDFIKPDEWKALIG-GKKIRPGLVIEVAPD-FKVEVL 117
T+V+PARL+G K TG +VE++L D+W+ L K+++ G V+ K
Sbjct: 61 TRVLPARLFGTKEDTGAKVELLLLKQETGDKWETLAKPAKRVKKGTVVTFGDGRLKAICT 120
Query: 118 EHIEEGKFRVKLLGEEPL-KLIDKYGHIPIPPYLKREEEPIDRVYYQTIFAKEKGAVASP 176
E +E G +++ + ++++ G +P+PPY+K + + D+ YQT+++KE G+ A+P
Sbjct: 121 EELEHGGRKMEFQYDGIFYEVLESLGEMPLPPYIKEQLD--DKERYQTVYSKEIGSAAAP 178
Query: 177 TASLHFSXXXXXXXXXXGINFAFITLHVSYGTFKPVKVERVEEHRVDPEYXXXXXXXXXX 236
TA LHF+ G+ FITLHV GTF+PV + VEEH + E+
Sbjct: 179 TAGLHFTEEILQQLKDKGVQIEFITLHVGLGTFRPVSADEVEEHNMHAEFYQMSEETAAA 238
Query: 237 XXXXXXXXXXXXXXXXXXXXXLET------KPFEPFEGWTDLYIYPGFKFKVVDAMITNF 290
LET F+ GWT ++IYPG++FK +D MITNF
Sbjct: 239 LNKVRENGGRIISVGTTSTRTLETIAGEHDGQFKASSGWTSIFIYPGYEFKAIDGMITNF 298
Query: 291 HLPRSSLLILVSAFAGREFILKAYREAVEKRYRFYSYGDGMLIL 334
HLP+SSL++LVSA AGRE IL+AY AVE+ YRF+S+GD MLI+
Sbjct: 299 HLPKSSLIMLVSALAGRENILRAYNHAVEEEYRFFSFGDAMLII 342
gb|AAD53918.1|AF179611_2 (AF179611) S-adenosylmethionine tRNA ribosyltransferase [Zymomonas
mobilis]
Length = 342
Score = 239 bits (604), Expect = 2e-62
Identities = 131/343 (38%), Positives = 199/343 (57%), Gaps = 12/343 (3%)
Query: 1 MRIEEFDYELPEELIAKYPAVPRHSARLMVLNRKDQSIKHDTFINLPDYLEEGDLLVFNN 60
MR++ FD+ELP E IA PAVPR SARL+ + ++ H +LP+ L+EGDLL+FN+
Sbjct: 1 MRVDLFDFELPTESIALRPAVPRESARLLQVTSQEMDDYH--VADLPNLLQEGDLLIFND 58
Query: 61 TKVIPARLYGRKPTGGRVEVVLTDFIKPDEWKALI-GGKKIRPGLVIEVAPDFKVEVLEH 119
T+VIPA L G+K R+ + +W+A + K+++ +++ D V+
Sbjct: 59 TRVIPAYLEGKK-NQARISANMIKREGLRQWQAFVRNAKRLKSDDIVDFGQDVSARVVHR 117
Query: 120 IEEGKFRVKLLGEEPLK-LIDKYGHIPIPPYL--KREEEPIDRVYYQTIFAKEKGAVASP 176
++G + LG EP++ L+++ G++P+PPY+ KR + DR YQTIFA+++GAVA+P
Sbjct: 118 NDDGSVLFEFLGNEPVEILLERAGNMPLPPYIAHKRPADQQDRQDYQTIFAEKEGAVAAP 177
Query: 177 TASLHFSXXXXXXXXXXGINFAFITLHVSYGTFKPVKVERVEEHRVDPEYXXXXXXXXXX 236
TASLHF+ G+ +TLHV GTF P+KVE ++H + E+
Sbjct: 178 TASLHFTEELLQRLEEKGVKHTKVTLHVGAGTFLPMKVEDSDDHVMHSEWGQISAETANA 237
Query: 237 XXXXXXXXXXXXXXXXXXXXXL-----ETKPFEPFEGWTDLYIYPGFKFKVVDAMITNFH 291
+ ET PF TD++I PG++F+V+D ++TNFH
Sbjct: 238 INETKKQGGRVIATGTTSLRLIDSAADETGLVHPFSDETDIFITPGYRFRVIDGLMTNFH 297
Query: 292 LPRSSLLILVSAFAGREFILKAYREAVEKRYRFYSYGDGMLIL 334
LP+S+L +LVSA GRE +L AY+ A++ YRFYSYGD L+L
Sbjct: 298 LPKSTLFMLVSALMGREKMLAAYQHAIDTGYRFYSYGDSSLLL 340
sp|Q55850|QUEA_SYNY3 S-ADENOSYLMETHIONINE:TRNA RIBOSYLTRANSFERASE-ISOMERASE (QUEUOSINE
BIOSYNTHESIS PROTEIN QUEA) >gi|1001768|dbj|BAA10606|
(D64004) queuosine biosynthesis protein QueA
[Synechocystis sp.]
Length = 366
Score = 230 bits (580), Expect = 1e-59
Identities = 133/353 (37%), Positives = 198/353 (55%), Gaps = 25/353 (7%)
Query: 3 IEEFDYELPEELIAKYPAVPRHSARLMVLNRKDQ-SIKHDTFINLPDYLEEGDLLVFNNT 61
+ +DY LP + IA+ PAVPR +RL+V+ KD ++ H F +L +YL+ GDLLV N+T
Sbjct: 7 LSSYDYHLPADCIAQTPAVPRDHSRLLVV--KDPFTVDHQHFYDLGNYLKPGDLLVLNDT 64
Query: 62 KVIPARLYGRKPTGGRVEVVLTDFIKPDEWKALIG-GKKIRPGLVIEVA------PDFKV 114
KV+PARL+G+K G VE++L ++W AL+ GK+++ G ++ PD
Sbjct: 65 KVMPARLHGQKENGVAVEILLLTECTENQWIALVKPGKRLKLGAKVKFPARDGEKPDLWA 124
Query: 115 EVLEHIEEGKFRVKLLGEEPLK----LIDKYGHIPIPPYLKREEEPIDRVYYQTIFAKEK 170
+L+ + R+ EP + ++ YG IP PPY+ + D+ YQTI+A++
Sbjct: 125 TILQRDDATGGRLLEFVLEPGQRLWDVLPDYGEIPFPPYIGDRQATNDQ--YQTIYAEKL 182
Query: 171 GAVASPTASLHFSXXXXXXXXXXGINFAFITLHVSYGTFKPVKVERVEEHRVDPEYXXXX 230
GAVA+PTA LHF+ G+ A +TLHV GTF+PV+ E + +H + E+
Sbjct: 183 GAVAAPTAGLHFTEELFTKLAQQGVQTAKVTLHVGIGTFRPVEAENILDHVMHQEWIDVP 242
Query: 231 XXXXXXXXXXXXXXXXXXXXXXXXXXXLE---------TKPFEPFEGWTDLYIYPGFKFK 281
LE +P +PF G TDL+IYPG++++
Sbjct: 243 PTTVEVIEKTRASGGRIFAVGTTVVRCLEGMAQESDFPRQPLQPFRGQTDLFIYPGYQWR 302
Query: 282 VVDAMITNFHLPRSSLLILVSAFAGREFILKAYREAVEKRYRFYSYGDGMLIL 334
+VD +ITNFHLP+SSLL+LVSA R ++ Y A+ K YRFYS+GD M+IL
Sbjct: 303 LVDGLITNFHLPKSSLLMLVSALISRPRLMAIYETAIAKGYRFYSFGDAMVIL 355
gi|4154891 (AE001471) S-ADENOSYLMETHIONINE TRNA RIBOSYLTRANSFERASE-ISOMERASE
[Helicobacter pylori J99]
Length = 345
Score = 220 bits (554), Expect = 2e-56
Identities = 134/344 (38%), Positives = 190/344 (54%), Gaps = 16/344 (4%)
Query: 3 IEEFDYELPEELIAKYPAVPRHSARLMVLNRKDQSIKHDTFINLPDYLEEGDLLVFNNTK 62
+E +DY LP+ELIA YP +P+ A+L+V R+ Q+I H TF ++ D+ + L+V N+TK
Sbjct: 6 LESYDYHLPKELIANYPVLPKEKAKLLVYERRSQTITHTTFEHVLDFFPKNALVVLNDTK 65
Query: 63 VIPARLYGRKPT---GGRVEVVLTDFIKPDEWKALIGGKKIRPGLVIEVAPDFKVEVLEH 119
VI ARL+G K EV F K + I G KI+ G I ++ EVLE
Sbjct: 66 VIKARLFGSKHAFLPSKTTEVFFHRFFKGNTALTQIKG-KIKVGDKIFFDANYYAEVLEL 124
Query: 120 IEEGK-------FRVKLLGEEPLKLIDKYGHIPIPPYLKREEEPIDRVYYQTIFAKEKGA 172
+ G+ + L E LKL+++YGH+P+PPY+KR +E +D YQ++FAK GA
Sbjct: 125 LHNGQRLIAFYDNQTPLNQENILKLLEQYGHMPLPPYIKRADESLDAHEYQSVFAKHIGA 184
Query: 173 VASPTASLHFSXXXXXXXXXXGINFAFITLHVSYGTFKPVKVERVEEHRVDPE--YXXXX 230
VA+PTASLHFS AF+TLHV GTF V+ + + EH++ E +
Sbjct: 185 VAAPTASLHFS-QNALEKLLKDFKHAFLTLHVGAGTFLSVETKDIREHQIHTEVLHIPKK 243
Query: 231 XXXXXXXXXXXXXXXXXXXXXXXXXXXLETKPFEPFEGWTDLYIYPGFKFKVVDAMITNF 290
L+ E FE D++++ + V+ ++TNF
Sbjct: 244 SQEILQESQEVLCIGTTALRSVEYFKRLKNPNQESFE--CDIFLHLANPIQHVNHLLTNF 301
Query: 291 HLPRSSLLILVSAFAGREFILKAYREAVEKRYRFYSYGDGMLIL 334
HLP+SSLL+LVSA G E + Y+ A+EK+YRFYSYGDGMLIL
Sbjct: 302 HLPKSSLLMLVSAMIGLEKTKEIYKIAIEKKYRFYSYGDGMLIL 345
sp|O25702|QUEA_HELPY S-ADENOSYLMETHIONINE:TRNA RIBOSYLTRANSFERASE-ISOMERASE (QUEUOSINE
BIOSYNTHESIS PROTEIN QUEA) >gi|2314205|gb|AAD08102.1|
(AE000613) S-adenosylmethionine:tRNA
ribosyltransferase-isomerase (queA) [Helicobacter pylori
26695]
Length = 345
Score = 219 bits (552), Expect = 3e-56
Identities = 134/344 (38%), Positives = 186/344 (53%), Gaps = 16/344 (4%)
Query: 3 IEEFDYELPEELIAKYPAVPRHSARLMVLNRKDQSIKHDTFINLPDYLEEGDLLVFNNTK 62
+E +DY LP+ELIA YP +P+ A+L+V R+ Q I H TF ++ D+ + L+V N+TK
Sbjct: 6 LESYDYYLPKELIASYPVLPKEKAKLLVYERRSQKITHTTFEHVLDFFPKNALIVLNDTK 65
Query: 63 VIPARLYGRKPT---GGRVEVVLTDFIKPDEWKALIGGKKIRPGLVIEVAPDFKVEVLEH 119
VI ARL+G K EV F K + I G KI+ G I ++ EVLE
Sbjct: 66 VIKARLFGSKHAFLPSKTTEVFFHRFFKNNTALTQIKG-KIKVGDKIFFDANYHAEVLEL 124
Query: 120 IEEGK-------FRVKLLGEEPLKLIDKYGHIPIPPYLKREEEPIDRVYYQTIFAKEKGA 172
+ G+ + L E LKL+++YGH+P+PPY+KR +E +D YQ++FAK GA
Sbjct: 125 LHNGQRLIAFYDNKTPLNQENILKLLEQYGHMPLPPYIKRADESLDAHEYQSVFAKHMGA 184
Query: 173 VASPTASLHFSXXXXXXXXXXGINFAFITLHVSYGTFKPVKVERVEEHRVDPEYXX--XX 230
VA+PTASLHFS AF+TLHV GTF V+ + + EH++ E
Sbjct: 185 VAAPTASLHFS-QNTLEKLLKDFKHAFLTLHVGAGTFLSVETKDIREHQIHTEVLRIPKK 243
Query: 231 XXXXXXXXXXXXXXXXXXXXXXXXXXXLETKPFEPFEGWTDLYIYPGFKFKVVDAMITNF 290
LE E FE D++++ V+ ++TNF
Sbjct: 244 SQEILQKSQEILCVGTTALRSVEYFKRLENPNQEAFE--CDIFLHFANPILHVNYLLTNF 301
Query: 291 HLPRSSLLILVSAFAGREFILKAYREAVEKRYRFYSYGDGMLIL 334
HLP+SSLL+LVS G E + Y+ A+EK+YRFYSYGDGMLIL
Sbjct: 302 HLPKSSLLMLVSTMIGLEKTKEIYKTAIEKKYRFYSYGDGMLIL 345
emb|CAA14676| (AJ235270) S-ADENOSYLMETHIONINE:tRNA RIBOSYLTRANSFERASE-ISOMERASE
(queA) [Rickettsia prowazekii]
Length = 383
Score = 190 bits (477), Expect = 2e-47
Identities = 121/396 (30%), Positives = 189/396 (47%), Gaps = 80/396 (20%)
Query: 1 MRIEEFDYELPEELIAKYPAVPRHSARLMVLNRKDQSIKHDTFINLPDYLEEGDLLVFNN 60
M++ +FD+ LP LIA+YP+ R ++ L++ K K F N+ DYL++GDLLVFNN
Sbjct: 1 MKLSDFDFNLPSALIAQYPSSERDNSDLLIAGTKHIKTK---FYNIIDYLKKGDLLVFNN 57
Query: 61 TKVIPARLYGRKPTGGRVEVVLTDFIKPDEWKALIGGKKIRPGLVIEVAPDF-----KVE 115
+KVI A+L+ G + + L + + W A +P + + +F K+
Sbjct: 58 SKVIKAKLH----LGKNITINLNKKLSDNCWIAF-----AKPARKLNIGDEFYFDTHKII 108
Query: 116 VLEHIEEGKFRVKLLGE--EPLKLIDKYGHIPIPPYLKRE-------------------- 153
+ E + G+ +VK + + +K +DKYG IP+P Y+KR
Sbjct: 109 ITEKLAIGEIKVKFMLDNISIIKFLDKYGEIPLPFYIKRPSPVCYSNMALCCKPENTLKI 168
Query: 154 ---------------------------EEPIDRVYYQTIFAKEKGAVASPTASLHFSXXX 186
+ D YQTI+++ +G+VA+PTA LHF+
Sbjct: 169 KSIPHNMSKIVTNNSNTVNLRSNDGIIDSTNDNDRYQTIYSQIEGSVAAPTAGLHFTKNI 228
Query: 187 XXXXXXXGINFAFITLHVSYGTFKPVKVERVEEHRVDPEYXXXXXXXXXXXXXXXXXXXX 246
G++ AF+TLHV GTF PVK E + EH++ EY
Sbjct: 229 LDKLKTKGVHTAFVTLHVGAGTFLPVKTENIHEHKMHTEYCSITTETAEIINKTKQEGRS 288
Query: 247 XXXXXXXXXXXLETK---------PFEPFEGWTDLYIYPGFKFKVVDAMITNFHLPRSSL 297
+E+ FE TD++I PGF F+VVD ++TNFH P+S+L
Sbjct: 289 IIAVGTTTLRTIESACNNGIVRAGNFE-----TDIFITPGFNFQVVDMLLTNFHFPKSTL 343
Query: 298 LILVSAFAGREFILKAYREAVEKRYRFYSYGDGMLI 333
IL+ AFAG + + Y+ A++++ RF+SYGD L+
Sbjct: 344 FILICAFAGFKEMHALYKYAIKEKMRFFSYGDATLL 379
gb|AAF11138.1|AE002001_4 (AE002001) s-adenosylmethionine:tRNA ribosyltransferase-isomerase
[Deinococcus radiodurans]
Length = 362
Score = 182 bits (458), Expect = 3e-45
Identities = 124/343 (36%), Positives = 180/343 (52%), Gaps = 23/343 (6%)
Query: 8 YELPEELIAKYPAVPRHSARLMVLNRKDQSIKHDTFINLPDYLEEGDLLVFNNTKVIPAR 67
++LPE+ IA+ A PR ++RLMV+ + IKH F +LPD L DLLVFN ++VIPAR
Sbjct: 20 FDLPEDRIAQTGAEPRDTSRLMVVG---EQIKHRMFRDLPDLLRPSDLLVFNESRVIPAR 76
Query: 68 LYGRKPT-----GGRVEVVLT--DF-IKPDEWKALIGGKKIRPG----LVIEVAPDFKVE 115
+ RKP GG+VEV+L +F + + W A + K R G L A + E
Sbjct: 77 VLARKPVVNGFGGGQVEVLLLREEFDVGANVWSAYLKPAK-RAGHELWLGENEATGHRAE 135
Query: 116 VLEHIEEGKFRVKLLGEEPLKLIDKYGHIPIPPYLKR-EEEPIDRVYYQTIFAKEKGAVA 174
V+ +++G R+ + +D+ G +P+PPY+ + + R YQT++AK G+VA
Sbjct: 136 VVGVLDDGA-RLLRFDHDIKPHLDEIGRLPLPPYINAGDSDETWRERYQTVYAKTPGSVA 194
Query: 175 SPTASLHFSXXXXXXXXXXGINFAFITLHVSYGTFKPVKVERVEEHRVDPEYXXXXXXXX 234
+PTA LHF+ G+ A +TLHV GTFKP++ V +H + E
Sbjct: 195 APTAGLHFTPELLARLDEMGVERASVTLHVGAGTFKPIQGP-VADHVMHAERYEVSETNA 253
Query: 235 XXXXXXXXXXXXXXXXXXXXXXXLETK----PFEPFEGWTDLYIYPGFKFKVVDAMITNF 290
LE+ G T ++I PG + +V D +ITN
Sbjct: 254 AAITRAKAEGRRVVAVGTTTVRTLESAWDGAAVRAGAGETRIFITPGTRVEVPDLLITNL 313
Query: 291 HLPRSSLLILVSAFAGREFILKAYREAVEKRYRFYSYGDGMLI 333
HLP S+LL+LV+AFAG + I AY A+ + YRFYS GD ML+
Sbjct: 314 HLPGSTLLLLVAAFAGEDRIRAAYDAALSQDYRFYSLGDAMLL 356
emb|CAA13168| (AJ231126) z63f [Vibrio cholerae]
Length = 202
Score = 115 bits (286), Expect = 4e-25
Identities = 63/177 (35%), Positives = 105/177 (58%), Gaps = 7/177 (3%)
Query: 11 PEELIAKYPAVPRHSARLMVLNRKDQSIKHDTFINLPDYLEEGDLLVFNNTKVIPARLYG 70
P YP R ++RL+ L+ + TF ++ + +E GDLLVFNNT+VIPAR++G
Sbjct: 20 PRRADCSYPKAERTASRLLQLDGNSGQLVDGTFKDVFELVEPGDLLVFNNTRVIPARMFG 79
Query: 71 RKPTGGRVEVVLTDFIKPDEWKALI---GGKKIRPGLVIEVAPDFKVE-VLEHIEEGKFR 126
RK +GG++EV++ + DE L K +PG + + + + V++ + F
Sbjct: 80 RKASGGKLEVLVERML--DEHTILAHVRSSKPPKPGTELYLGENDEFHAVMQARHDALFE 137
Query: 127 VKLLGEE-PLKLIDKYGHIPIPPYLKREEEPIDRVYYQTIFAKEKGAVASPTASLHF 182
++ E L ++++ GH+P+PPY+ R +E D+ YQT++ ++ GAVA+PTA LHF
Sbjct: 138 IRFTAETVVLDILNQIGHMPLPPYIDRPDEETDKERYQTVYNQKPGAVAAPTAGLHF 194
sp|O51053|QUEA_BORBU PUTATIVE S-ADENOSYLMETHIONINE:TRNA RIBOSYLTRANSFERASE-ISOMERASE
(QUEUOSINE BIOSYNTHESIS PROTEIN QUEA) >gi|2687901
(AE001116) S-adenosylmethionine: tRNA
ribosyltransferase-isomerase [Borrelia burgdorferi]
Length = 215
Score = 104 bits (258), Expect = 8e-22
Identities = 70/223 (31%), Positives = 110/223 (48%), Gaps = 20/223 (8%)
Query: 1 MRIEEFDYELPEELIAKYPAVPRHSARLMVLNRKDQSIKHDTFINLPDYLEEGDLLVFNN 60
M+ +EF + L LI +YP+ R S+RL+VL+ + Q I H +N + L+ + +FNN
Sbjct: 4 MKTKEFHFNLSHSLIEQYPSEKRGSSRLIVLDPQLQKIYHKNSVN--NILKYINSNIFNN 61
Query: 61 TKVIPARLYGRKPTGGRVEVVLTDFIKPDEWKALIGGKKIR---------PGLVIEVAPD 111
+K +R+Y VE ++ D I + + AL+ K + GL+ E+
Sbjct: 62 SKARKSRIYAESEMSSNVEFLILDRIDTNLFTALVSKSKKQIIGNFYKFPEGLMDEILSK 121
Query: 112 FKVEVLEHIEEGKFRVKLLGEEPLKLIDKYGHIPIPPYLKREEEPIDRVYYQTIFAKEKG 171
EV+ +GE+ +K+ +P+P Y+KR+ + ID YQTI++K G
Sbjct: 122 NSSEVVLKFNNN------VGED---YFEKHCFVPLPSYIKRDYDKIDEDRYQTIYSKYVG 172
Query: 172 AVASPTASLHFSXXXXXXXXXXGINFAFITLHVSYGTFKPVKV 214
+ AS TA LHFS I + FITLHV F K+
Sbjct: 173 STASATAGLHFSRDLFSAFENNNIEYDFITLHVGAWDFSSCKI 215
emb|CAA70098.1| (Y08885) S-adenosylmethionine: tRNA ribosyltransferase-isomerase
[Borrelia burgdorferi]
Length = 108
Score = 59.7 bits (142), Expect = 3e-08
Identities = 34/99 (34%), Positives = 57/99 (57%), Gaps = 2/99 (2%)
Query: 1 MRIEEFDYELPEELIAKYPAVPRHSARLMVLNRKDQSIKHDTFINLPDYLEEGDLLVFNN 60
M+ +EF + LP LI +YP+ R S+RL+VL+ + Q I H+ +N + L+ + +FNN
Sbjct: 1 MKTKEFHFNLPHSLIEQYPSEKRGSSRLIVLDPQLQKIYHENSVN--NILKYINSNIFNN 58
Query: 61 TKVIPARLYGRKPTGGRVEVVLTDFIKPDEWKALIGGKK 99
+KV +R+Y VE ++ D I + + AL+ K
Sbjct: 59 SKVRKSRIYVESEMSSNVEFLILDRIGTNLFTALVSKSK 97
sp|O25549|RUVA_HELPY HOLLIDAY JUNCTION DNA HELICASE RUVA >gi|2314020|gb|AAD07932.1|
(AE000598) Holliday junction DNA helicase (ruvA)
[Helicobacter pylori 26695]
Length = 183
Score = 36.0 bits (81), Expect = 0.48
Identities = 27/97 (27%), Positives = 50/97 (50%), Gaps = 17/97 (17%)
Query: 26 ARLMVLNRKDQSIKHDTFINLPDYLEEGDLLVFNNTKVIPARLYGRKPTGGRVEVVLTDF 85
ARL +L Q IK D + L +LEEG+ ++F RL GGR+ + +
Sbjct: 44 ARLKIL----QVIKEDAHL-LYGFLEEGEKILFE-------RLLKINGVGGRIALAILSS 91
Query: 86 IKPDEWKALIGGKKIR-----PGLVIEVAPDFKVEVL 117
P+E++++I K+++ PG+ ++A V+++
Sbjct: 92 FSPNEFESIIATKEVKRLQQVPGIGKKLADKIMVDLI 128
emb|CAA15008| (AJ235272) PROBABLE 3-HYDROXYACYL-COA DEHYDROGENASE B0272.3 (fadB)
[Rickettsia prowazekii]
Length = 720
Score = 35.2 bits (79), Expect = 0.82
Identities = 31/108 (28%), Positives = 50/108 (45%), Gaps = 13/108 (12%)
Query: 38 IKHDTFINLPDYLEEGDLLVFNNTKVIPAR-LYGRKPTGGRVEVVLTDFIKPDEWKALIG 96
IKH + + YL+E D ++ +NT +P + L P + V+T F P + L+
Sbjct: 100 IKHQLYNKIIPYLKE-DAIIASNTSTLPLKKLKVNLPNNIKSRFVITHFFNPPRYMELV- 157
Query: 97 GKKIRPGLVIEVAPDFKVEVLEHIEEGKFRVKLLGEEPLKLIDKYGHI 144
L+I+ K EV+E + F K+LG+ +K D G I
Sbjct: 158 ------ELIID--HTIKDEVIEKV--SVFLTKMLGKTIIKCNDTPGFI 195
sp|Q9ZKW9|RUVA_HELPJ HOLLIDAY JUNCTION DNA HELICASE RUVA >gi|4155386 (AE001511) HOLLIDAY
JUNCTION DNA HELICASE [Helicobacter pylori J99]
Length = 183
Score = 32.5 bits (72), Expect = 5.4
Identities = 26/97 (26%), Positives = 48/97 (48%), Gaps = 17/97 (17%)
Query: 26 ARLMVLNRKDQSIKHDTFINLPDYLEEGDLLVFNNTKVIPARLYGRKPTGGRVEVVLTDF 85
ARL +L Q IK D + L +LEE + ++F RL GGR+ + +
Sbjct: 44 ARLKIL----QVIKEDAHL-LYGFLEESEKILFE-------RLLKINGVGGRIALAILSS 91
Query: 86 IKPDEWKALIGGKKIR-----PGLVIEVAPDFKVEVL 117
P+E++ +I K+++ PG+ ++A V+++
Sbjct: 92 FSPNEFENIIATKEVKRLQQVPGIGKKLADKIMVDLI 128
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
Posted date: Jan 12, 2000 2:26 PM
Number of letters in database: 136,724,657
Number of sequences in database: 444,417
Lambda K H
0.322 0.144 0.426
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84522008
Number of Sequences: 444417
Number of extensions: 3315265
Number of successful extensions: 6326
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 6274
Number of HSP's gapped (non-prelim): 21
length of query: 334
length of database: 136,724,657
effective HSP length: 52
effective length of query: 282
effective length of database: 113,614,973
effective search space: 32039422386
effective search space used: 32039422386
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 70 (31.7 bits)
