Score E
Sequences producing significant alignments: (bits) Value
gi|2983619 (AE000726) histidyl-tRNA synthetase [Aquifex aeo... 705 0.0
sp|Q58406|SYH_METJA HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 145 5e-34
sp|O28631|SYH_ARCFU HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 120 2e-26
sp|O26346|SYH_METTH HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 114 1e-24
sp|O58028|SYH_PYRHO HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 109 3e-23
emb|CAB50581.1| (AJ248288) histidyl-tRNA synthetase (hisS) ... 106 3e-22
gb|AAD36166.1|AE001768_15 (AE001768) histidyl-tRNA syntheta... 102 5e-21
gi|2459964 (AF010189) HisX [Pseudomonas stutzeri] 101 1e-20
gi|2982856 (AE000674) histidyl-tRNA synthetase [Aquifex aeo... 98 9e-20
gi|2618864 (AF017113) histidyl-tRNA synthetase [Bacillus su... 89 8e-17
dbj|BAA79634.1| (AP000060) 438aa long hypothetical histidyl... 87 2e-16
gb|AAC68145.1| (AE001325) Histidyl tRNA Synthetase [Chlamyd... 86 4e-16
sp|P56455|SYH_HELPY HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 83 5e-15
gi|4155714 (AE001539) HISTIDYL-TRNA SYNTHETASE [Helicobacte... 80 3e-14
sp|O32039|SYH_BACSU HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 79 5e-14
sp|Q55653|SYH1_SYNY3 PROBABLE HISTIDYL-TRNA SYNTHETASE 1 (H... 79 7e-14
sp|Q55267|SYH_SYNP7 HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 79 7e-14
sp|P51348|SYH_PORPU HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 76 3e-13
sp|O32422|SYH_STAAU HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 76 5e-13
sp|P74592|SYH2_SYNY3 PROBABLE HISTIDYL-TRNA SYNTHETASE 2 (H... 76 5e-13
gi|3659909 (AF020715) histidyl-tRNA synthetase [Arabidopsis... 75 8e-13
sp|Q02147|YHI3_LACLA HYPOTHETICAL 38.0 KD PROTEIN IN HISC-H... 75 1e-12
sp|P30053|SYH_STREQ HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 72 7e-12
pir||S30232 histidine--tRNA ligase (EC 6.1.1.21) - Streptoc... 72 7e-12
sp|P56194|SYH_THETH HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 71 2e-11
sp|P12081|SYH_HUMAN HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 68 1e-10
sp|O83647|SYH_TREPA HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 68 1e-10
ref|NP_032240.1|| histidyl tRNA synthetase >gi|2501005|sp|Q... 68 2e-10
sp|O51160|SYH_BORBU HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 66 5e-10
pdb|1HTT|A Chain A, Histidyl-Trna Synthetase >gi|1942449|pd... 66 5e-10
sp|P04804|SYH_ECOLI HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 66 5e-10
gi|2896164 (AF047040) histidyl-tRNA synthetase; aminoacyl-t... 66 7e-10
gb|AAF10919.1|AE001981_4 (AE001981) histidyl-tRNA synthetas... 65 1e-09
sp|P93422|SYH_ORYSA HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 65 1e-09
ref|NP_015358.1|HTS1| cytoplasmic and mitochondrial histidi... 64 1e-09
pir||SYBYHC histidine--tRNA ligase (EC 6.1.1.21), cytosolic... 64 1e-09
gi|171732 (M14048) histidine-tRNA synthetase mitochondrial ... 64 1e-09
gb|AAD18802| (AE001648) Histidyl tRNA Synthetase [Chlamydia... 63 3e-09
gi|4103152 (AF020716) histidyl-tRNA synthetase [Triticum ae... 63 3e-09
sp|Q9ZDL9|SYH_RICPR HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 63 4e-09
sp|Q50641|SYH_MYCTU HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 62 8e-09
gi|4103154 (AF020717) histidyl-tRNA synthetase [Triticum ae... 61 2e-08
sp|P43823|SYH_HAEIN HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 60 4e-08
gb|AAF11015.1|AE001989_3 (AE001989) histidyl-tRNA synthetas... 59 9e-08
emb|CAA19087.1| (AL023591) histidyl-tRNA synthetase [Mycoba... 57 3e-07
sp|P46696|SYH_MYCLE HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 57 3e-07
emb|CAA17892.1| (AL022103) histidyl-trna synthetase, mitoch... 57 3e-07
sp|P49590|SYHH_HUMAN HISTIDYL-TRNA SYNTHETASE HOMOLOG (HIST... 56 6e-07
sp|P47281|SYH_MYCGE HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 52 1e-05
sp|P46220|SYH_SULSH PUTATIVE HISTIDYL-TRNA SYNTHETASE (HIST... 49 7e-05
sp|P07178|SYH_MESAU HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 46 5e-04
gb|AAD36120.1|AE001764_23 (AE001764) histidyl-tRNA syntheta... 46 6e-04
sp|P75069|SYH_MYCPN HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 45 0.001
sp|P70076|SYH_FUGRU HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 45 0.001
emb|CAA93416.1| (Z69384) predicted using Genefinder; Simila... 39 0.063
ref|NP_002100.1|| histidyl-tRNA synthetase >gi|32458|emb|CA... 39 0.063
emb|CAA83774| (Z33137) His-tRNA synthetase [Mycoplasma capr... 39 0.063
sp|P50511|RP32_VIBCH RNA POLYMERASE SIGMA-32 FACTOR >gi|116... 36 0.41
ref|NP_014178.1|POP1| Component of nuclear RNase P and RNas... 35 0.93
gi|1477704 (U57659) spiralin [Spiroplasma kunkelii] 34 2.1
pir||G64502 hypothetical protein MJ1626 - Methanococcus jan... 33 3.6
sp|P53000|PROA_CAMJE GAMMA-GLUTAMYL PHOSPHATE REDUCTASE (GP... 33 3.6
gb|AAD07664.1| (AE000573) H. pylori predicted coding region... 33 4.7
sp|P55112|YPD6_CAEEL HYPOTHETICAL ZINC METALLOPROTEINASE C0... 33 4.7
gb|AAD14008|S68230_1 (S68230) histidyl tRNA synthetase [Cae... 32 6.2
emb|CAA22914| (AL035309) hypothetical protein [Homo sapiens] 32 6.2
pir||S41763 histidyl tRNA synthetase - Caenorhabditis elegans 32 6.2
sp|P34183|SYH_CAEEL HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TR... 32 6.2
pir||S41762 histidine--tRNA ligase (EC 6.1.1.21) - Caenorha... 32 6.2
emb|CAB60375.1| (AL110485) Y46G5A.6 [Caenorhabditis elegans] 32 8.1
gi|2983619 (AE000726) histidyl-tRNA synthetase [Aquifex aeolicus]
Length = 383
Score = 705 bits (1801), Expect = 0.0
Identities = 361/383 (94%), Positives = 361/383 (94%)
Query: 1 MKTSLISGERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGSKE 60
MKTSLISGERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGSKE
Sbjct: 1 MKTSLISGERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGSKE 60
Query: 61 AITFKDSYTKESFGLRLDFTTQVVRTISHLRNVKLPERVYYFGKVFSLDRRGFEKLQTGV 120
AITFKDSYTKESFGLRLDFTTQVVRTISHLRNVKLPERVYYFGKVFSLDRRGFEKLQTGV
Sbjct: 61 AITFKDSYTKESFGLRLDFTTQVVRTISHLRNVKLPERVYYFGKVFSLDRRGFEKLQTGV 120
Query: 121 ELIGEKSILADFEVIQVLTEFLKSLGLKDLKVILSHAGIVRKVADEREELLRAFSERNLE 180
ELIGEKSILADFEVIQVLTEFLKSLGLKDLKVILSHAGIVRKVADEREELLRAFSERNLE
Sbjct: 121 ELIGEKSILADFEVIQVLTEFLKSLGLKDLKVILSHAGIVRKVADEREELLRAFSERNLE 180
Query: 181 ALKEVLGNDIKFFVEVSKNPEVLNFXXXXXXXXXXXXXXXXXXXXXXFGIDYAYDLGEVR 240
ALKEVLGNDIKFFVEVSKNPEVLNF FGIDYAYDLGEVR
Sbjct: 181 ALKEVLGNDIKFFVEVSKNPEVLNFLEKYDLEKEKEELLELGKLLEEFGIDYAYDLGEVR 240
Query: 241 NFPYYTGVIFEIYELKSGKALAGGGRYDNLSKIYGKEYPATGGAVYLERLLDILPKNVEK 300
NFPYYTGVIFEIYELKSGKALAGGGRYDNLSKIYGKEYPATGGAVYLERLLDILPKNVEK
Sbjct: 241 NFPYYTGVIFEIYELKSGKALAGGGRYDNLSKIYGKEYPATGGAVYLERLLDILPKNVEK 300
Query: 301 KDYFVIDKTKKRLGLKLADVLREKGKKVGMEIVKERGLEHSLIYAFDKGFKEVLLVEDEI 360
KDYFVIDKTKKRLGLKLADVLREKGKKVGMEIVKERGLEHSLIYAFDKGFKEVLLVEDEI
Sbjct: 301 KDYFVIDKTKKRLGLKLADVLREKGKKVGMEIVKERGLEHSLIYAFDKGFKEVLLVEDEI 360
Query: 361 VKVYTTPKDYVVMKIREFLDLIE 383
VKVYTTPKDYVVMKIREFLDLIE
Sbjct: 361 VKVYTTPKDYVVMKIREFLDLIE 383
sp|Q58406|SYH_METJA HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|2127969|pir||G64424 histidine--tRNA ligase (EC
6.1.1.21) - Methanococcus jannaschii >gi|1591660
(U67542) histidyl-tRNA synthetase (hisS) [Methanococcus
jannaschii]
Length = 416
Score = 145 bits (363), Expect = 5e-34
Identities = 132/395 (33%), Positives = 200/395 (50%), Gaps = 34/395 (8%)
Query: 8 GERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEG-SKEAITFKD 66
G R + EE + + K ++FE +GY I FE +E GE K+ FKD
Sbjct: 10 GTRDFLPEEMKKRRFVENKLREVFERYGYKEILTPTFESFELIAKKTGEEIRKQLYVFKD 69
Query: 67 SYTKESFGLRLDFTTQVVR-TISHLRNVKLPERVYYFGKVFSLDR----RGFEKLQTGVE 121
+E LR + T+ VVR ++ L+N++ P R+YYF F +R R E Q G E
Sbjct: 70 HGGRE-MALRPEMTSPVVRFYLNELKNLQKPLRLYYFANCFRYERPQAGRFREFWQMGCE 128
Query: 122 LIGEKSILADFEVIQVLTEFLKSLGLKDLKVILSHAGIVR------KVADEREELLRAFS 175
LIG K LAD EV+ + + L ++GL D V + H G+++ V++E E +R
Sbjct: 129 LIGCKEPLADAEVLNLAMDGLINIGL-DFDVHIGHLGVLKGVLEKFNVSEEEEVKIRRLI 187
Query: 176 ER----NLEA-LKEVLGNDIKFFV-EVSK---NPEVLN-FXXXXXXXXXXXXXXXXXXXX 225
++ NL+ L ++LG + K + E+ K + EVL+
Sbjct: 188 DKEDYDNLKIYLTQILGEEKKELIFEILKFKGSREVLDELKEILKDFPKSMEAINNLEEI 247
Query: 226 XXFGI--DYAYDLGEVRNFPYYTGVIFEIYELKSGKALAGGGRYDNLSKIYGKE-YPATG 282
F I Y +LG R YYTG++FEIY K K + GGGRYDNL + +G E PA G
Sbjct: 248 LEFVIHDKYTINLGIARGLDYYTGMVFEIYGKKGAKQICGGGRYDNLIETFGGEPTPAVG 307
Query: 283 GAVYLER-LLDILPKNVEKKDYFVI----DKTKKRLGLKLADVLREKGKKVGMEIVKERG 337
A +R +++I ++E++ +I DK + L +AD LR+ GK V +EI+ R
Sbjct: 308 FAYGFDRIMMNIDDLDIEEESILIIPVKKDKELIKKSLIIADKLRKAGKIVELEIM-GRK 366
Query: 338 LEHSLIYAFDKGFKEVLLV-EDEIVKVYTTPKDYV 371
L +L YA +GFK+V++V E E+ + T KD +
Sbjct: 367 LRKALDYANSRGFKKVIIVGEKELNEGKVTVKDMI 401
sp|O28631|SYH_ARCFU HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|2648908 (AE000989) histidyl-tRNA synthetase (hisS)
[Archaeoglobus fulgidus]
Length = 409
Score = 120 bits (298), Expect = 2e-26
Identities = 110/384 (28%), Positives = 172/384 (44%), Gaps = 27/384 (7%)
Query: 1 MKTSLISGERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGS-K 59
MK G R + +E +EI K+ KI E+FGY + FE E GEG +
Sbjct: 1 MKIERPRGTRDFLPDEMERRREIEKRMRKIAESFGYREVATPTFEYLELFTRKSGEGIIE 60
Query: 60 EAITFKDSYTKESFGLRLDFTTQVVRT-ISHLRNVKLPERVYYFGKVFSLDR----RGFE 114
E FKD ++ LR + T V+R ++ + P R YYF F +R R E
Sbjct: 61 EMYVFKDKSGRD-LALRPELTAPVMRMFVNECSVMPKPLRFYYFANCFRYERPQKGRYRE 119
Query: 115 KLQTGVELIGEKSILADFEVIQVLTEFLKSLGLKDLKVILSHAGIVRKVA-----DEREE 169
Q GVELIG +S LAD EVI + + LK +G+ + + + H GI+R + D +
Sbjct: 120 FWQFGVELIGSESYLADAEVIILADKILKDVGV-NFSLEIGHVGIMRHLLKPIGEDRASK 178
Query: 170 LLRAFSERNLEALKEVLGN--------DIKFFVEVSKNPEVLNFXXXXXXXXXXXXXXXX 221
++R + + E L+ L D F + K E +
Sbjct: 179 VMRLIDKGDREGLESYLAEIRVNEDLRDKIFSLLELKGDESVIEEAKEIIDYDFGHLESL 238
Query: 222 XXXXXXFGIDYAYDLGEVRNFPYYTGVIFEIYE--LKSGKALAGGGRYDNLSKIYGKEYP 279
G+D+ +LG R YYTGV+FE Y L + K + GGG Y+ S G P
Sbjct: 239 SALLRDVGVDFTLNLGIARGLDYYTGVVFECYAEGLGAQKQVCGGGSYELSSLFGGPVTP 298
Query: 280 ATGGAVYLERLLDILPKNVEKKDYFVIDKTK--KRLGLKLADVLREKGKKVGMEIVKERG 337
+TG A+ +R+ + +K + K + ++A +LRE+G ++++ R
Sbjct: 299 STGFAIGFDRVCEACSVEAGEKSVVAVVSFKGLESQAFRVASMLRERGFTAVVDVM-GRN 357
Query: 338 LEHSLIYAFDKGFK-EVLLVEDEI 360
L+ + +A + G K V+L DE+
Sbjct: 358 LKKQMSFASEMGVKYAVILGPDEV 381
sp|O26346|SYH_METTH HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|2621293 (AE000811) histidyl-tRNA synthetase
[Methanobacterium thermoautotrophicum]
Length = 444
Score = 114 bits (283), Expect = 1e-24
Identities = 121/406 (29%), Positives = 174/406 (42%), Gaps = 40/406 (9%)
Query: 1 MKTSLISGERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGSKE 60
M S G R + E KE+ + FE +GY I FE + + GE
Sbjct: 20 MDISRPRGTRDFLFAEMRNRKEVEGVLRRTFETYGYHEIKTPIFEELKLFTVKSGEEVVN 79
Query: 61 AITFKDSYTKESFGLRLDFTTQVVRT-ISHLRNVKLPERVYYFGKVFSLDR----RGFEK 115
I LR + T V R ++ ++ P ++YYFG F +R R +
Sbjct: 80 QIYHFTDKGGRELALRPELTAPVARLYMNEMQKKPKPIKMYYFGSCFRYERPQAGRFRQF 139
Query: 116 LQTGVELIGEKSILADFEVIQVLTEFLKSLGLKDLKVILSHAGIVRKVADEREELLRAFS 175
Q G ELIG +S LA+ EVI + +E L LGL +V + H GI+R + REE+
Sbjct: 140 WQFGCELIGGRSPLAEAEVISLASESLHELGLDGFEVHIGHLGILRGILG-REEIPSELQ 198
Query: 176 ER-----------NLEALK---EVLGNDIKFFVEV---SKNPEVLN-----FXXXXXXXX 213
+R LEAL EV G + +++ PEVL+
Sbjct: 199 DRIMGVIDKGDVEELEALLGEVEVSGESRELLMKLIGTQGGPEVLDDVEEYLGGYSESLE 258
Query: 214 XXXXXXXXXXXXXXFGID-YAYDLGEVRNFPYYTGVIFEIY--ELKSGKALAGGGRYDNL 270
FG+D Y +LG R YYTG++FEIY EL + K + GGG Y+ +
Sbjct: 259 ALEEFRELVETLHYFGVDDYHVNLGIARGLDYYTGIVFEIYVPELGAQKQICGGGTYNLV 318
Query: 271 SKIYGKEYPATGGAVYLERLLDIL---PKNVEKKDYFVI---DKTKKRLGLKLADVLREK 324
G+ +TG A +RL++ L +N+ D FVI D T +++ LR
Sbjct: 319 ETFGGERVESTGFAFGFDRLMNALGMDEENMRMVDVFVIPIQDSTVPE-AIRITQELRAA 377
Query: 325 GKKVGMEIVKERGLEHSLIYAFDKGFK-EVLLVEDEIVKVYTTPKD 369
G +E V R L +L +A + G + VL E EI T KD
Sbjct: 378 GVSADLE-VSGRKLRKALSHADNLGARFAVLTGEREIQDGKVTVKD 422
sp|O58028|SYH_PYRHO HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|3256679|dbj|BAA29362.1| (AP000001) 431aa long
hypothetical histidyl-tRNA synthetase [Pyrococcus
horikoshii]
Length = 431
Score = 109 bits (271), Expect = 3e-23
Identities = 118/395 (29%), Positives = 179/395 (44%), Gaps = 50/395 (12%)
Query: 6 ISGERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGS-KEAITF 64
+ G R + E+ + +F+K ++FEA+G+ I E + +L GE K+ F
Sbjct: 5 VKGTRDFLPEDMAKRRWVFEKIREVFEAYGFKEILTPVMEYTKLFQLRSGEEVVKQLYAF 64
Query: 65 KDSYTKESFGLRLDFTTQVVRT-ISHLRNVKLPERVYYFGKVFSLDR----RGFEKLQTG 119
KD ++ LR D T+ V R ++ + P + YY +F + R E Q G
Sbjct: 65 KDKGGRD-VSLRPDMTSSVARLYVNSFQMAPKPIKWYYIANMFRYEEPQSGRYREFWQAG 123
Query: 120 VELIGEKSILADFEVIQVLTEFLKSLGLKDLKVILSH-------AGIV--------RKVA 164
VELIG + AD EVI + E S GL+D V + AG++ ++
Sbjct: 124 VELIGSDKVEADAEVIALFVESYLSTGLRDFTVNIGDRILLDEFAGMLGVSDDIGLMRII 183
Query: 165 DEREELLRAFSERNLEALKEV-LGND----IKFFVEVSKNPEVL-----NFXXXXXXXXX 214
D+R++L + E + LKE LG + VE+ P+ +
Sbjct: 184 DKRDKLSQ---EEFINLLKEFGLGESEIEKVLELVEIKGRPDNVLPLAEELFKSKKAKEE 240
Query: 215 XXXXXXXXXXXXXFGID--YAYDLGEVRNFPYYTGVIFEIY---ELKSGKALAGGGRYDN 269
+G+ DLG R F YYT ++FE EL G ++ GGGRYDN
Sbjct: 241 ISKLYKLTDLLEWYGVKDWIRIDLGIARGFDYYTSIVFEAISPNELGIG-SIGGGGRYDN 299
Query: 270 LSKIYG-KEYPATGGAVYLERLLDIL------PKNVEKKDYFVID-KTKKRLGLKLADVL 321
L +I+G K PATG A+ +ERLL IL PK + FVI K +++ + +A L
Sbjct: 300 LIEIFGGKPTPATGFAIGIERLLPILEWKGLIPKPQTGPEVFVIPLKDMEKVAINIAVKL 359
Query: 322 REKGKKVGMEIVKERGLEHSLIYAFDKGFKEVLLV 356
R + K +E+ R L +L YA G K V++V
Sbjct: 360 RREKIKTDIEL-SGRKLGKALDYANRVGAKLVIIV 393
emb|CAB50581.1| (AJ248288) histidyl-tRNA synthetase (hisS) [Pyrococcus abyssi]
Length = 431
Score = 106 bits (262), Expect = 3e-22
Identities = 121/434 (27%), Positives = 187/434 (42%), Gaps = 67/434 (15%)
Query: 6 ISGERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGS-KEAITF 64
+ G R + EE + +F+K ++FE +G+ + E + +L GE K+ F
Sbjct: 5 VKGTRDFLPEEMVKRRWVFEKIREVFETYGFKEVLTPVMEYTKLFQLRSGEEVVKQLYAF 64
Query: 65 KDSYTKESFGLRLDFTTQVVRT-ISHLRNVKLPERVYYFGKVFSLDR----RGFEKLQTG 119
KD ++ LR D T+ V R ++ + P + YY +F + R E Q G
Sbjct: 65 KDKGGRD-VALRPDMTSSVARLYVNSFQTAPKPIKWYYIANMFRYEEPQSGRYREFWQAG 123
Query: 120 VELIGEKSILADFEVIQVLTEFLKSLGLKDL------KVILSHAGIVRKVADE------- 166
VELIG I AD EVI + + S GLKD +V+L + V D+
Sbjct: 124 VELIGSDKIEADAEVIALFVDSYLSTGLKDFTVNIGDRVLLDEFAKMLGVKDDIGLMRII 183
Query: 167 -------REELLRAFSERNLE--ALKEVLGNDIKFFVEVSKNPEVL-----NFXXXXXXX 212
+EE L+A E L+ +++VL +E+ P+ +
Sbjct: 184 DKKDKLSQEEFLKALGEFGLDENGIEKVLN-----LIEIKGKPDDVLPLAEELFTSERAK 238
Query: 213 XXXXXXXXXXXXXXXFGID--YAYDLGEVRNFPYYTGVIFEIY---ELKSGKALAGGGRY 267
+ +D DLG R F YYT ++FE +L G ++ GGGRY
Sbjct: 239 EEISRLYNLVDILSWYEVDEWIQIDLGIARGFDYYTSIVFEAIVPNDLGIG-SIGGGGRY 297
Query: 268 DNLSKIY-GKEYPATGGAVYLERLLDI------LPKNVEKKDYFVIDKTKKR-LGLKLAD 319
DNL +++ GK PATG A+ +ERL+ I LP+ D FVI R + +
Sbjct: 298 DNLIEVFGGKPTPATGFAIGIERLIPILEWKGLLPELKAGPDVFVIPVGDSRDVATAIVT 357
Query: 320 VLREKGKKVGMEIVKERGLEHSLIYAFDKGFKEVLLV-----EDEIVKV--------YTT 366
LR+ G + +E+ R L +L YA G + ++V E +V +
Sbjct: 358 RLRKAGIRSDIEL-SGRKLRKALDYANRIGVRLSIIVGKRDLERGVVTIRDLESGNQVEV 416
Query: 367 PKDYVVMKIREFLD 380
P D VV K+RE L+
Sbjct: 417 PVDNVVTKVRELLN 430
gb|AAD36166.1|AE001768_15 (AE001768) histidyl-tRNA synthetase [Thermotoga maritima]
Length = 420
Score = 102 bits (252), Expect = 5e-21
Identities = 117/398 (29%), Positives = 181/398 (45%), Gaps = 51/398 (12%)
Query: 20 VKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGS----KEAITFKDSYTKESFGL 75
V+E F+ + E+ G + I FE E + GE S KE TF+D + S L
Sbjct: 23 VEETFRN---VCESAGIEEIRTPIFEQTELFVRSVGEESDIVQKEMYTFQDKAGR-SITL 78
Query: 76 RLDFTTQVVRTI--SHLRNVKLPERVYYFGKVFSLDRRGFEKL----QTGVELIGEKSIL 129
R + T VVR + L + +R YY G +F ++ +L Q G E+IG +S
Sbjct: 79 RPEGTAPVVRAFLENSLIDRGFQQRYYYIGPMFRYEKPQSGRLRQFHQVGFEIIGPESPK 138
Query: 130 ADFEVIQVLTEFLKSLGLKDLKVILSHAG--IVRKVADEREELLRAFSERNLEALKEVLG 187
ADFEVI ++ FL+ LGL K+ L+ G + RK E L+ + R L+ L +
Sbjct: 139 ADFEVIMLVDTFLRRLGLTKYKIHLNSIGCPVCRK---NYREALKEYYGRILDNLCDDCK 195
Query: 188 N----------DIKFFVEVSKN-PEVLNFXXXXXXXXXXXXXXXXXXXXXXFGIDYAYDL 236
D K E S N P+ +++ F I+Y D
Sbjct: 196 RRYETNILRLLDCKVDHEYSLNAPKSVDYLCDSCRAHYKKLKEYLNT----FEIEYVEDH 251
Query: 237 GEVRNFPYYTGVIFEIYE--LKSGKALAGGGRYDNL-SKIYGKEYPATGGAVYLERL--- 290
VR YYT +FE+ L + A+AGGGRYD L +++ G PA G A +ER+
Sbjct: 252 TLVRGLDYYTRTVFEVRHEGLGAQSAIAGGGRYDGLFAELGGSSVPALGFAGGIERIILA 311
Query: 291 -----LDILPKNVEKKDYFVIDKTKKRLGLKLADVLREKGKKVGMEIVKERGLEHSLIYA 345
++I KNV + + G++LA LR+KG V ++I+ +R L L +A
Sbjct: 312 LKAEGIEIPMKNVHLVYIATLGEKAFMDGVRLAGELRKKGLSVDVDIM-DRKLSGQLKHA 370
Query: 346 FDKGFKEVLLVEDEIVKVYTTPKDYVVMKIREFLDLIE 383
G + +++ DE ++ K V+++ E D +E
Sbjct: 371 SRMGSRYAVIIGDEELE-----KGIVILRDLETGDQVE 403
gi|2459964 (AF010189) HisX [Pseudomonas stutzeri]
Length = 395
Score = 101 bits (248), Expect = 1e-20
Identities = 79/307 (25%), Positives = 135/307 (43%), Gaps = 31/307 (10%)
Query: 16 ESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGSK-EAITFK--DSYTKES 72
E+ ++ ++ + +F+ +GY+ + H E E L G G + TFK D +
Sbjct: 20 EAARIETARRRVLDLFQRWGYELVITPHVEFLE--SLLSGSGQDLDLKTFKVIDPLSGRQ 77
Query: 73 FGLRLDFTTQVVRTISHLRNVKLPERVYYFGKVFSLDRRGF----EKLQTGVELIGEKSI 128
GLR D T QV R +H + P R+ Y G V R +Q G EL G+ S
Sbjct: 78 MGLRADITPQVARVDAHTLRREGPSRLCYAGSVLHAKPRALATSRSPIQLGAELYGDSST 137
Query: 129 LADFEVIQVLTEFLKSLGLKDLKVILSHAGIVRKVA-------DEREELLRAFSERNLEA 181
+D EVI ++ E L+ + D+ + L H GI R +A + + L A + ++
Sbjct: 138 SSDIEVISLMLEMLELAMVPDVHMDLGHVGIYRGLARAAGLSGEAEQRLFDAMQRKAMDE 197
Query: 182 LKEV-------LGNDIKFFVEVSKNPEVLNFXXXXXXXX---XXXXXXXXXXXXXXFGID 231
+ E+ L ++ + E L+ +
Sbjct: 198 IAELTATVEPSLAAMLQALARLCGGRETLDAARRVLAEAPAPVTEALEALIRIADQLALR 257
Query: 232 YA-----YDLGEVRNFPYYTGVIFEIYELKSGKALAGGGRYDNLSKIYGKEYPATGGAVY 286
Y +DLGE+R + Y+TGV+F ++ G+++A GGRYD++ +G+ PATG +
Sbjct: 258 YPDLPLYFDLGELRGYHYHTGVVFAVFVPGVGQSIAQGGRYDDIGADFGRARPATGFSTD 317
Query: 287 LERLLDI 293
L+ L+ +
Sbjct: 318 LKTLVSL 324
gi|2982856 (AE000674) histidyl-tRNA synthetase [Aquifex aeolicus]
Length = 403
Score = 98.3 bits (241), Expect = 9e-20
Identities = 106/381 (27%), Positives = 173/381 (44%), Gaps = 32/381 (8%)
Query: 15 EESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGS----KEAITFKDSYTK 70
+++ ++I A KI + + ++ I L E E + + GE + KE F+D +
Sbjct: 15 KDAKKFRKISDTARKILKLYNFEEIILPVVEYAELFQRSVGETTDIVQKEMFVFEDRKGR 74
Query: 71 ESFGLRLDFTTQVVRT-ISHLRNVKLPE-RVYYFGKVFSLDR----RGFEKLQTGVELIG 124
+ LR + T VR I H P +++Y G +F +R R + Q G E+ G
Sbjct: 75 K-LALRPEGTAGTVRAFIQHKLYALRPYVKLFYEGPMFRYERPQAGRYRQFHQIGAEVFG 133
Query: 125 EKSILADFEVIQVLTEFLKSLGLKDLKVILSHAGIVRKVADEREELLRAFSERNLEALKE 184
AD E+I+++ + L++LG+K + V ++ G + RE LL + E +
Sbjct: 134 VAEPHADAEIIKIVYDILQALGIKGVVVEINSLGCKKDREAYREALLNYLTGVKEELCSD 193
Query: 185 VLG----NDIKFF--------VEVSKNPEVLNFXXXXXXXXXXXXXXXXXXXXXXFGIDY 232
+ N ++ V V + P++++F F +Y
Sbjct: 194 CISRMDRNPLRVLDCKVETCKVAVREAPKMIDFLCDECREHYEKLKNYLKALDIPFRENY 253
Query: 233 AYDLGEVRNFPYYTGVIFEIYELKSGKALAGGGRYDNL-SKIYGKEYPATGGAVYLERLL 291
VR YYT +FE + G L GGRYD L ++ G PA G A+ +ERL+
Sbjct: 254 NL----VRGLDYYTRTVFEAVSDELGLTLIAGGRYDYLVEELGGPPTPALGFALGVERLM 309
Query: 292 DILPKNVEKKD-YFVID-KTKKRLGLKLADVLREKGKKVGMEIVKERGLEHSLIYAFDKG 349
+LP EK++ YFVI L++AD+LR+KGK V ++ GL+ L +A G
Sbjct: 310 LLLPDEEEKEEVYFVIPFGDVHEYALRVADILRKKGKVVEYS-YRKGGLKKQLEFADKLG 368
Query: 350 FK-EVLLVEDEIVKVYTTPKD 369
K V++ EDE+ T KD
Sbjct: 369 VKYAVIIGEDEVKNQEVTIKD 389
gi|2618864 (AF017113) histidyl-tRNA synthetase [Bacillus subtilis]
>gi|2636006|emb|CAB15498| (Z99121) histidyl-tRNA
synthetase [Bacillus subtilis]
Length = 391
Score = 88.5 bits (216), Expect = 8e-17
Identities = 86/340 (25%), Positives = 140/340 (40%), Gaps = 30/340 (8%)
Query: 21 KEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGSKEAITFKDSYTKESFGLRLDFT 80
K++ + + + +GY ++ E Y+ + ++ D K + LR D T
Sbjct: 22 KKVRRSLTDLIDKWGYQFMETPTLEFYDTVGVQSAIEEQQLFKLLDQDGK-TLVLRPDMT 80
Query: 81 TQVVRTI-SHLRNVKLPERVYYFGKVFSLDRRG----FEKLQTGVELIGEKSILADFEVI 135
+ R S L P RV Y VF R E Q GVELIG+ + AD EVI
Sbjct: 81 GPIARVAASKLLKHGHPLRVGYAANVFRAQEREGGRPAEFEQVGVELIGDGTTSADAEVI 140
Query: 136 QVLTEFLKSLGLKDLKVILSHAGIVRKVADE------REELLRAFS-ERNLEALKEVLGN 188
++ LK+ GL K+ + HAGI + E R ++LR F E+N +E + +
Sbjct: 141 ALVVGALKNAGLASFKIAIGHAGIADALFVEVLGNVERADVLRRFLYEKNYVGYREHVKS 200
Query: 189 ---------------DIKFFVEVSKNPEVLNFXXXXXXXXXXXXXXXXXXXXXXFGIDYA 233
+++ +EV E + +
Sbjct: 201 LPLSSIDKSRLLELLELRGGIEVCGRAEEIVDSAQGKSVVDELKALWDILEDYGCTENVR 260
Query: 234 YDLGEVRNFPYYTGVIFEIYELKSGKALAGGGRYDNLSKIYGKEYPATGGAVYLERLLDI 293
DL V + YYTG++FE+Y G + GGRY+ L + PATG + ++RL++
Sbjct: 261 LDLNMVSHMSYYTGILFEVYAENVGFVIGSGGRYNKLLGHFDSPAPATGFGLRIDRLIEA 320
Query: 294 LPKNVE--KKDYFVIDKTKKRLGLKLADVLREKGKKVGME 331
L E + D + K ++ + A+ R KG KV ++
Sbjct: 321 LHMKDEPCEIDAVIFSKEQRAQAIAYANEERMKGNKVVLQ 360
dbj|BAA79634.1| (AP000060) 438aa long hypothetical histidyl-tRNA synthetase
[Aeropyrum pernix]
Length = 438
Score = 87.4 bits (213), Expect = 2e-16
Identities = 88/371 (23%), Positives = 165/371 (43%), Gaps = 43/371 (11%)
Query: 8 GERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGSKEAIT--FK 65
G R + E L K + + ++FE++G+D ++ E +E +GE ++ + F+
Sbjct: 14 GFRDFPPEVMILRKRLLSRLERVFESYGFDPLDTPAVEYWETLSGKYGEEAESRLIWRFQ 73
Query: 66 DSYTKESFGLRLDFTTQVVRTISHLRNVKLPERVYYFGKVFSLDR----RGFEKLQTGVE 121
D ++ + LR D T + R ++ + +P + Y+ GKV+ + R E +Q +
Sbjct: 74 DPWSGRWYALRYDLTVPLARFVASNPGLPMPFKRYHIGKVWRHEEPQKGRYREFVQCDAD 133
Query: 122 LIGEKSILADFEVIQVLTEFLKSLGLKDLKVILSHAGIVRKVADER------EELLRAFS 175
++G AD E++ + + + +LGL +V ++ ++ V +E + RA
Sbjct: 134 IVGSPYPEADAEIVSLAVDSIDALGLPGFRVRVNDRRLLAGVFEEELGFDNPLPIYRAID 193
Query: 176 E----------RNLEALKEVLGNDIKFFVEV----SKNPEVLNFXXXXXXXXXXXXXXXX 221
+ R LE L + G+ ++ +E+ + EVL
Sbjct: 194 KLDKIGVEGVRRELERL-GLPGSTVERIMEIISWRGRPGEVLESVRRGYGSNEKVREALD 252
Query: 222 XXXXXXFGID---YAYDLGEVRNFPYYTGVIFEIY--ELKSGKALAGGGRYDNLSKIY-G 275
D D+ VR YYTG I E+ E + G ++AGGGRYD L ++ G
Sbjct: 253 HLEEMLSLADDKRVELDMSLVRGLDYYTGPILEVVLDEPRIG-SVAGGGRYDGLVGMFAG 311
Query: 276 KEYPATGGAVYLERLLD---------ILPKNVEKKDYFVIDKTKKRLGLKLADVLREKGK 326
++ PATG ++ +ER++D + K V + V+D+ R + A +LR G
Sbjct: 312 RDIPATGVSIGIERIIDAGLELGVYSLDVKTVAQVAVVVLDRRYYRYAWEAARILRRGGL 371
Query: 327 KVGMEIVKERG 337
V +++ + G
Sbjct: 372 NVRIDLSRASG 382
gb|AAC68145.1| (AE001325) Histidyl tRNA Synthetase [Chlamydia trachomatis]
Length = 428
Score = 86.2 bits (210), Expect = 4e-16
Identities = 87/301 (28%), Positives = 135/301 (43%), Gaps = 28/301 (9%)
Query: 17 SNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGS----KEAITFKDSYTKES 72
S+L K + A +I +G+D I FE E L GE S KE TF D + S
Sbjct: 26 SSLWKRVEHAAHRICNLYGFDEIRTPVFEKTE-TFLRVGEYSDIVKKEVYTFLDK-KRRS 83
Query: 73 FGLRLDFTTQVVRTI-SHLRNVKLPERVYYFGKVFSLDR----RGFEKLQTGVELIGEKS 127
LR + T VVR + H +++ + YY +F +R R + Q G+E IG +
Sbjct: 84 LTLRPEGTAAVVRALLDHSADMRKDNKFYYILPMFRYERQQSGRYRQHHQFGLEAIGVRH 143
Query: 128 ILADFEVIQVLTEFLKSLGLKDLKVILSHAGIVRKVADEREELLRAFSERNLEALKEVLG 187
L D EV+ +L +F ++GL+ +++ ++ G +K +E LR F ++L+ L +
Sbjct: 144 PLRDAEVLSLLWDFYAAVGLQHMQIHVNFLG-GQKTRARYDEALREFFRKDLDRLSPL-- 200
Query: 188 NDIKFFVEV-----SKNPEVLNFXXXX------XXXXXXXXXXXXXXXXXXFGIDYAYDL 236
+ ++ + SK PE F GI +A +
Sbjct: 201 SQERYHANLLRILDSKEPEDQEFIEKAPSILDYIDDRDLSYFDAVLAQLKALGISFAINP 260
Query: 237 GEVRNFPYYTGVIFEIYEL--KSGKALAGGGRYDNL-SKIYGKEYPATGGAVYLERLLDI 293
VR YYT ++FE + + AL GGGRYD L ++ G PA G V LER++
Sbjct: 261 RLVRGLDYYTDLVFEAVTVVGEHSYALGGGGRYDELVAQSGGPSMPAFGFGVGLERVIQT 320
Query: 294 L 294
L
Sbjct: 321 L 321
sp|P56455|SYH_HELPY HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|2314351|gb|AAD08236.1| (AE000625) histidyl-tRNA
synthetase (hisS) [Helicobacter pylori 26695]
Length = 442
Score = 82.7 bits (201), Expect = 5e-15
Identities = 94/395 (23%), Positives = 166/395 (41%), Gaps = 57/395 (14%)
Query: 22 EIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGS----KEAITFKDSYTKESFGLRL 77
++ K +F++FG+ I H E + + + S KE FKD ++ LR
Sbjct: 23 QLLAKVSVVFQSFGFVPIETPHLE---YAQTLLPDASSDIQKEIYRFKDHGDRD-VALRF 78
Query: 78 DFTTQVVRTIS-HLRNVKLPERVYYFGKVFSLDR----RGFEKLQTGVELIGEKSILADF 132
D T + R +S H + + +P + Y G VF +R R E Q + IG +S++ D
Sbjct: 79 DLTVPLARFVSLHHQTLGMPFKRYAIGNVFRGERAQKGRYREFTQCDFDFIGSESLVCDA 138
Query: 133 EVIQVLTEFLKSLGLKDLKVILSHAGIVRKVAD-----EREELLRAFSERNL-------E 180
E+IQV+ LK+L L+D V ++H I+ + + + E LR + E
Sbjct: 139 EIIQVIVASLKALDLEDFCVSINHRKILNGICEYFGISQVNEALRIVDKLEKIGLNGVEE 198
Query: 181 ALKEVLGNDIKFFVEVSKNPEVLNFXXXXXXXXXXXXXXXXXXXXXXFGID--------- 231
LK+ G + E+ + ++ GI
Sbjct: 199 ELKKECGLNSNTIKELLELIQIKQNDLSHAEFFEKIAYLKDYNENLKKGIQDLERLYQLL 258
Query: 232 ---------YAYDLGEVRNFPYYTGVIFE--IYELKSGKALAGGGRYDNLSKIYGKE-YP 279
Y D R YYTG+++E + E+KS ++ GGRYD+L+K + KE
Sbjct: 259 GDLQISQNLYKIDFSIARGLGYYTGIVYETTLNEMKSLGSVCSGGRYDHLTKNFSKENLQ 318
Query: 280 ATGGAVYLERL------LDILPKNVEKKDYFVIDKTKKRLGL--KLADVLREKGKKVGME 331
G ++ ++RL + +L + + + ++ +LA+ LR+ G + E
Sbjct: 319 GVGASIGIDRLIVALSEMQLLDERSTQAKVLIACMHEEYFSYANRLAESLRQSG--IFSE 376
Query: 332 IVKE-RGLEHSLIYAFDKGFKEVLLVEDEIVKVYT 365
+ E + ++ YA KG + V ++ +E K T
Sbjct: 377 VYPEAQKIKKPFSYANHKGHEFVAVIGEEEFKSET 411
gi|4155714 (AE001539) HISTIDYL-TRNA SYNTHETASE [Helicobacter pylori J99]
Length = 442
Score = 80.0 bits (194), Expect = 3e-14
Identities = 94/398 (23%), Positives = 168/398 (41%), Gaps = 63/398 (15%)
Query: 22 EIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGS----KEAITFKDSYTKESFGLRL 77
++ K +F++FG+ I H E + + + S KE FKD ++ LR
Sbjct: 23 QLLAKVSVVFQSFGFVPIETPHLE---YAQTLLPDASSDIQKEIYRFKDHGDRD-VALRF 78
Query: 78 DFTTQVVRTIS-HLRNVKLPERVYYFGKVFSLDR----RGFEKLQTGVELIGEKSILADF 132
D T + R +S H + + +P + Y G VF +R R E Q + IG +S++ D
Sbjct: 79 DLTVPLARFVSLHHQILGMPFKRYAIGNVFRGERAQKGRYREFTQCDFDFIGSESLVCDA 138
Query: 133 EVIQVLTEFLKSLGLKDLKVILSHAGI---------------VRKVADEREELLRAFSER 177
E+IQV+ LK+L L+D V ++H I V ++ D+ E++ E
Sbjct: 139 EIIQVIIASLKALDLEDFCVSINHRKILNGICEYFGIAQVNEVLRIVDKLEKIGLNGVEE 198
Query: 178 NLEALKEVLGNDIKFFVEVSKNPEVLNFXXXXXXXXXXXXXXXXXXXXXXFGID------ 231
L+ ++ N IK +E+ ++ GI
Sbjct: 199 ELKKECDLDSNTIKDLLEM---VQIKQNDLSHAEFFEKIAYLKDYNENLKKGIQDLERLY 255
Query: 232 ------------YAYDLGEVRNFPYYTGVIFE--IYELKSGKALAGGGRYDNLSKIYGKE 277
Y D R YYTG+++E + ++KS ++ GGRYD+L+K + KE
Sbjct: 256 QLLGDLQISQNLYKIDFSIARGLGYYTGIVYETTLNDMKSLGSVCSGGRYDHLTKNFSKE 315
Query: 278 -YPATGGAVYLERL------LDILPKNVEKKDYFVIDKTKKRLGL--KLADVLREKGKKV 328
G ++ ++RL + +L + + + ++ +LA+ LR+ G +
Sbjct: 316 NLQGVGASIGIDRLIVALSEMQLLDERSTQAKVLIACMHEEYFSYANRLAESLRQSG--I 373
Query: 329 GMEIVKE-RGLEHSLIYAFDKGFKEVLLVEDEIVKVYT 365
E+ E + ++ YA KG + V ++ +E K T
Sbjct: 374 FSEVYPEAQKIKKPFSYANHKGHEFVAVIGEEEFKSET 411
sp|O32039|SYH_BACSU HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|2635220|emb|CAB14715| (Z99118) histidyl-tRNA
synthetase [Bacillus subtilis]
Length = 424
Score = 79.2 bits (192), Expect = 5e-14
Identities = 97/357 (27%), Positives = 148/357 (41%), Gaps = 48/357 (13%)
Query: 20 VKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGS----KEAITFKDSYTKESFGL 75
V++I + + ++ Y I FE E GE + KE TF+D + S L
Sbjct: 23 VEQIMRDTCRTYQ---YKEIRTPIFEHTELFARGVGESTDIVQKEMYTFEDRKGR-SLTL 78
Query: 76 RLDFTTQVVRTISHLR---NVKLPERVYYFGKVFSLDR----RGFEKLQTGVELIGEKSI 128
R + T VR + + N P ++YY G +F +R R + Q G+E IG K
Sbjct: 79 RPEGTAAAVRAFNENKLFANPVQPTKLYYVGPMFRYERPQTGRYRQFYQFGIEAIGSKDP 138
Query: 129 LADFEVIQVLTEFLKSLGLKDLKVILSHAGIVRKVADEREELLRAFSERNLEALKEVLG- 187
D EV+ + + GL+++K++++ G RE L++ F R E +
Sbjct: 139 AIDAEVMALAMSIYEKAGLENVKLVINSLGDQDSRKSYREALVKHFEPRIEEFCSDCQSR 198
Query: 188 ---NDIKFF-------VEVSKN-PEVLNFXXXXXXXXXXXXXXXXXXXXXXFGIDYAYDL 236
N ++ E+ K+ P +L + GI Y D
Sbjct: 199 LHTNPLRILDCKKDRDHELMKSAPSILTY----LNEESAAYFEKVKQYLNDLGISYEIDP 254
Query: 237 GEVRNFPYYTGVIFEIYELKSG----KALAGGGRYDNL-SKIYGKEYPATGGAVYLERLL 291
VR YY FEI G LAGGGRYD L +I G E P G A+ +ERLL
Sbjct: 255 NLVRGLDYYNHTAFEIMSNAEGFGAITTLAGGGRYDGLVEQIGGPEAPGIGFAMSIERLL 314
Query: 292 DILPKNVEKKDYFVIDKTKKRLGLKLADV-LREKGKKVGMEIV---KERGLEHSLIY 344
+ + EK++ +DK G+ V L EK K + +V +E G+ + Y
Sbjct: 315 AAI--DAEKRE-LPVDK-----GIDCYIVTLGEKAKDYSVSLVYKLREAGISSEIDY 363
sp|Q55653|SYH1_SYNY3 PROBABLE HISTIDYL-TRNA SYNTHETASE 1 (HISTIDINE--TRNA LIGASE 1)
(HISRS 1) >gi|1001546|dbj|BAA10173| (D64000)
histidyl-tRNA synthetase [Synechocystis sp.]
Length = 447
Score = 78.8 bits (191), Expect = 7e-14
Identities = 110/415 (26%), Positives = 169/415 (40%), Gaps = 53/415 (12%)
Query: 6 ISGERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGS----KEA 61
I G R E+N + + A I + Y I FE E GE + KE
Sbjct: 7 IRGTRDILPPETNYWQWVEAIAKSILDRALYQEIRTPIFEQTSLFERGIGEATDVVGKEM 66
Query: 62 ITFKDSYTKESFGLRLDFTTQVVRTI--SHLRNVKLPERVYYFGKVFSLDR----RGFEK 115
+F D + LR + T VVR +L+ +R++Y G +F +R R +
Sbjct: 67 YSFTDRGDRP-ITLRPEGTAGVVRAYIEQNLQAAGGVQRLWYTGPMFRYERPQAGRQRQF 125
Query: 116 LQTGVELIGEKSILADFEVIQVLTEFLKSLGLKDLKVILSHAG-----------IVRKVA 164
Q GVE++G AD EVI + T+ LK+LGL +L + L+ G ++ +
Sbjct: 126 HQLGVEVLGSADPRADVEVIALGTDILKALGLSNLSLALNSVGNGGDRQRYREALIAYLT 185
Query: 165 DEREELLRAFSERNLEALKEVLGNDIKFFVEVSKN-PEVLNFXXXXXXXXXXXXXXXXXX 223
+ EL +R +L + K E+ ++ P +L+
Sbjct: 186 PFKAELDPDSQDRLERNPLRILDSKAKRTQEIVQDAPSILDHLGVDSQRHFDQVQQLLTN 245
Query: 224 XXXXFGIDYAYDLGEVRNFPYYTGVIFEIY--ELKSGKALAGGGRYDNL-SKIYGKEYPA 280
GI Y VR YYT FEI +L + + GGGRYD L +++ G PA
Sbjct: 246 ----LGIAYQLTPTLVRGLDYYTHTAFEIQSSDLGAQATVCGGGRYDGLVAELGGPVTPA 301
Query: 281 TGGAVYLERLLDILPK----NVEKKDYFVIDKTKKR--LGLKLADVLREKGKKVGMEIVK 334
G A+ LERL+ +L + D ++I K +K L LA LR +G V +++
Sbjct: 302 VGWAMGLERLIILLQQMATPPAPSPDLYLISKGEKAEPQALILAQKLRNQGLAVALDLSA 361
Query: 335 ERGLEHSLIYAFDKGFKEV--------LLVEDEIVKVYTTPKDYVVMKIREFLDL 381
AF K FK L++ D + T ++ K +E L L
Sbjct: 362 S---------AFGKQFKRADKSGAIACLVLGDGEIATGTVQLKWLADKAQETLQL 407
sp|Q55267|SYH_SYNP7 HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|530225 (L35476) histidyl tRNA synthetase
[Synechococcus sp.]
Length = 420
Score = 78.8 bits (191), Expect = 7e-14
Identities = 91/362 (25%), Positives = 146/362 (40%), Gaps = 47/362 (12%)
Query: 29 KIFEAFGYDYINLSHFEPYEFQELTFGEGSKEAITFKDSYTKESFGLRLDFTTQVVRT-I 87
++F+ +GY I E + + G + A+ S + GLR + T + R +
Sbjct: 29 QVFQQWGYQRIITPTLERLDTL-VAGGAVQRSAVIQVQSDEESGLGLRPELTASIARAAV 87
Query: 88 SHLRNVKLPERVYYFGKVFSLDRRGF-----EKLQTGVELIGEKSILADFEVIQVLTEFL 142
+ L LP R+YY VF +G E Q GVEL+G LAD EV+ VL + L
Sbjct: 88 TRLAGSSLPLRLYYLANVFRPAFQGDRLQQRELFQAGVELLGVGGTLADAEVLHVLADAL 147
Query: 143 KSLG-----LKDLKVILSHAGIVRKV-----ADEREELLRA---FSERNLEA-------- 181
LG L +++ A + R + D RE++ +A F LE+
Sbjct: 148 AELGFGQPPLGSWHLVVGEASLTRSLLQPFPKDLREKVRQAIAQFDRVTLESLPLESQLR 207
Query: 182 -----LKEVLGNDIKFFVEVSKNPEVLNFXXXXXXXXXXXXXXXXXXXXXXFGIDYAYDL 236
L ++ G + F ++ + L DL
Sbjct: 208 DRALLLHDLRGQPDQVFAKLQQ--LTLTPLEQTLRDRLAQLVELYNASAGPQDSPLLLDL 265
Query: 237 GEVRNFPYYTGVIFE-IYELKSGK-ALAGGGRYDNLSKIYGKE---YPATGGAVYLERLL 291
+R+F YYTG++FE +YE +G LA GGRYD L +Y + P G + +E L
Sbjct: 266 SLLRSFDYYTGIVFEVVYETPTGPWVLAQGGRYDRLLDVYDPQAAGQPGIGFSCNIENLQ 325
Query: 292 DI------LPKNVEKKDYFVIDKTKKRLGLKLADVLR-EKGKKVGMEIVKERGLEHSLIY 344
+ LP D VI + G LA+ R ++ ++ +E+ + ++
Sbjct: 326 QVLLAANRLPHRPPAIDQLVIPVDSEAYGAALAEAQRLQRQDQLRVELYLDSDRRPEVVQ 385
Query: 345 AF 346
AF
Sbjct: 386 AF 387
sp|P51348|SYH_PORPU HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|2147478|pir||S73269 histidine--tRNA ligase (EC
6.1.1.21) - Porphyra purpurea chloroplast >gi|1276814
(U38804) histidine tRNA synthetase [Porphyra purpurea]
Length = 430
Score = 76.5 bits (185), Expect = 3e-13
Identities = 96/413 (23%), Positives = 169/413 (40%), Gaps = 37/413 (8%)
Query: 2 KTSLISGERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGS--- 58
K I G + +E + I +K + + Y I FE + + GE +
Sbjct: 3 KIQAIRGTKDILPDEQLYWQFIHEKVASLLKYANYKEIKTPIFENSDLYDRGIGENTDIV 62
Query: 59 -KEAITFKDSYTKESFGLRLDFTTQVVRT-ISHLRNVKLP-ERVYYFGKVFSLDR----R 111
KE F D ++ LR + T VVR I + +V+ ++++Y G +F +R R
Sbjct: 63 NKEMYRFNDRSNRD-ITLRPEGTAGVVRAFIENRMDVQNRLQKLWYSGPMFRYERPQSGR 121
Query: 112 GFEKLQTGVELIGEKSILADFEVIQVLTEFLKSLGLKDLKVILSHAGIVRK----VADER 167
+ Q G+E IG AD E+I + L + DL++ ++ G A +
Sbjct: 122 QRQFHQLGIEFIGSSDARADAEIIHLAMNIFNDLSVNDLQLDINSIGKAEDRNHYQAKLQ 181
Query: 168 EELLRAFSERNLEALKEVLGNDIKFFVEVSK--------NPEVLNFXXXXXXXXXXXXXX 219
E L + + + + ++ + N I+ +K +P + +F
Sbjct: 182 EYLEQYYDDLDTDSQNRLSSNPIRILDTKNKYTQTILNDSPRISDF----LSLESEKHFD 237
Query: 220 XXXXXXXXFGIDYAYDLGEVRNFPYYTGVIFEIYELKS--GKALAGGGRYDNL-SKIYGK 276
+ Y + VR YY FEI LKS L GGGRYD+L ++ G
Sbjct: 238 DVCDYLTLLNVPYKINTQLVRGLDYYNDTAFEIKILKSQGQDTLCGGGRYDSLIHQLGGN 297
Query: 277 EYPATGGAVYLERLLDILPKNV----EKKDYFVIDK--TKKRLGLKLADVLREKGKKVGM 330
+ PA G A+ LERLL + N+ D +++ K +++G+ + LR + K +
Sbjct: 298 KTPAVGCAIGLERLLLVAKDNILLPHMSIDCYIVSKGIKARKIGIIITQFLRNQLVKTEL 357
Query: 331 EIVKERGLEHSLIYAFDKGFKEVLLVEDEIVKVYTTPKDYVVMKIREFLDLIE 383
+I L A K L++ D ++ T ++ + +E + +IE
Sbjct: 358 DI-SSSNFGKQLKQAHKKRAIACLILGDNEIQQETITIKWMYTQEQENMSIIE 409
sp|O32422|SYH_STAAU HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|2580436|dbj|BAA23141| (D76414) histidyl-tRNA
synthetase [Staphylococcus aureus]
Length = 420
Score = 76.1 bits (184), Expect = 5e-13
Identities = 94/378 (24%), Positives = 156/378 (40%), Gaps = 35/378 (9%)
Query: 15 EESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGS----KEAITFKDSYTK 70
E+S + I + ++ + Y I FE + G+ + KE TFKD +
Sbjct: 14 EDSKKWRYIENQLDELMTFYNYKEIRTPIFESTDLFARGVGDSTDVVQKEMYTFKDKGDR 73
Query: 71 ESFGLRLDFTTQVVRT-ISHLR--NVKLPERVYYFGKVFSLDRRGFEKL----QTGVELI 123
S LR + T VVR+ I H N P ++YY G +F +R+ + Q GVE I
Sbjct: 74 -SITLRPEGTAAVVRSYIEHKMQGNPNQPIKLYYNGPMFRYERKQKGRYRQFNQFGVEAI 132
Query: 124 GEKSILADFEVIQVLTEFLKSLGLKDLKVILSHAGIVRKVADEREELLRAFS----ERNL 179
G ++ D EV+ ++ +S GLK LK++++ G + + E L++ F E
Sbjct: 133 GAENPSVDAEVLAMVMHIYQSFGLKHLKLVINSVGDMASRKEYNEALVKHFEPVIHEFCS 192
Query: 180 EALKEVLGNDIKFF-VEVSKNPEVLNFX---XXXXXXXXXXXXXXXXXXXXXFGIDYAYD 235
+ + N ++ +V ++ E + GI Y D
Sbjct: 193 DCQSRLHTNPMRILDCKVDRDKEAIKTAPRITDFLNEESKAYYEQVKAYLDDLGIPYIED 252
Query: 236 LGEVRNFPYYTGVIFEIYELKSG-----KALAGGGRYDNLSKIY-GKEYPATGGAVYLER 289
VR YYT FE+ L GGGRY+ L ++ G G A+ +ER
Sbjct: 253 PNLVRGLDYYTHTAFELMMDNPNYDGAITTLCGGGRYNGLLELLDGPSETGIGFALSIER 312
Query: 290 LLDILPKN------VEKKDYFVIDKTKK--RLGLKLADVLREKGKKVGMEIVKERGLEHS 341
LL L + E D F++ + R +KL + LR G K + + +R ++
Sbjct: 313 LLLALEEEGIELDIEENLDLFIVTMGDQADRYAVKLLNHLRHNGIKADKDYL-QRKIKGQ 371
Query: 342 LIYAFDKGFKEVLLVEDE 359
+ A G K +++ D+
Sbjct: 372 MKQADRLGAKFTIVIGDQ 389
sp|P74592|SYH2_SYNY3 PROBABLE HISTIDYL-TRNA SYNTHETASE 2 (HISTIDINE--TRNA LIGASE 2)
(HISRS 2) >gi|1653789|dbj|BAA18700| (D90916) histidyl
tRNA synthetase [Synechocystis sp.]
Length = 401
Score = 76.1 bits (184), Expect = 5e-13
Identities = 80/331 (24%), Positives = 129/331 (38%), Gaps = 38/331 (11%)
Query: 31 FEAFGYDYINLSHFEPYEFQELTFGEGSKEAITFKDSYTKESFGLRLDFTTQVVRTISHL 90
F +GY I S E + + I +DS + GLR + T + R +
Sbjct: 31 FHRWGYQRIVTSTLEWLDTLVAGGAISANNVIQLQDS-GEGRLGLRPELTASIARAVVTR 89
Query: 91 RNVKLPERVYYFGKVFSLDRRGF-----EKLQTGVELIGEKSILADFEVIQVLTEFLKSL 145
P+R+ Y VF G+ E Q G+EL+ + AD E++ +LT+ L L
Sbjct: 90 MTDNQPQRLCYRANVFRNPPEGYHGKQMEFFQAGIELLFAGGVRADAEILLLLTDCLTQL 149
Query: 146 GLKDLKVILSHAGIVRKVADEREELLRAFSERNLEALKEVLGNDIKFFVEVSKNPEVLNF 205
GL D ++IL AG+ R + + L+A + L V + + + +KN + F
Sbjct: 150 GLSDWQLILGDAGLTRSLLAKLPPTLQATVRDCITRLDYVELSQLPYPDNEAKNLALQLF 209
Query: 206 XXXXXXXXXXXXXXXXXXXXXXFGI-------------------DYAYDLGEVRNFPYYT 246
G+ + DL ++ F YYT
Sbjct: 210 DLRGTVEEVLARLGKLDLQGECLGLVDRLQALLCLVAASGDGPANLVLDLSWLQPFDYYT 269
Query: 247 GVIFEIYELKSGK--ALAGGGRYDNLSKIY---GKEYPATGGAVYLERL------LDILP 295
G++F+ ++ L GGRYD L Y + +P TG ++ +E L L LP
Sbjct: 270 GMVFQAVSRQADNCYVLGQGGRYDQLLSQYHPQQQSFPGTGFSLNIEELHQCLLELGTLP 329
Query: 296 KNVEKKDYFV--IDKTKKRLGLKLADVLREK 324
+ DY V +D T + + A LR +
Sbjct: 330 TSTAPIDYLVCPVDDTAEGATFRHAQQLRRQ 360
gi|3659909 (AF020715) histidyl-tRNA synthetase [Arabidopsis thaliana]
Length = 486
Score = 75.3 bits (182), Expect = 8e-13
Identities = 85/381 (22%), Positives = 155/381 (40%), Gaps = 80/381 (20%)
Query: 8 GERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGSKEAITFKDS 67
G R + E+ L +F ++ +GY+ ++ E GE ++ + +
Sbjct: 74 GTRDFPPEDMRLRNWLFNHFKEVSRLYGYEEVDYPVLETEALFIRKAGEEIRDQLYCFED 133
Query: 68 YTKESFGLRLDFTTQVVR-TISHLRNVKLPERVYYFGKVFSLDR----RGFEKLQTGVEL 122
LR + T + R I ++V LP + + G+ + +R R E Q +++
Sbjct: 134 RGNRRVALRPELTPSLARLVIQKGKSVSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDI 193
Query: 123 IGEKSILADFEVIQVLTEFLKSLGL------------KDLKVILSHAGI----------- 159
IG + A+ E+I + F K +G+ K L+ +L G+
Sbjct: 194 IGVPQVTAEAELISSIVTFFKRIGITASDVGFKVSSRKVLQELLKKYGVPEDLFGRVCII 253
Query: 160 ---VRKVA---------------DEREELLRAFSERNLEALKEVLGNDIKFFVEVSKNPE 201
+ K+ D E+LL+ S ++L+ L++++G + ++ +
Sbjct: 254 IDKIEKIPIDEIKKELGFTGISEDAIEQLLQVLSVKSLDDLEDIIGGAGEAIADLKQ--- 310
Query: 202 VLNFXXXXXXXXXXXXXXXXXXXXXXFGID--YAYDLGEVRNFPYYTGVIFEIYELKSG- 258
FG +D VR YYTG++FE ++ K
Sbjct: 311 -------------------LFSLAEKFGYSEWIQFDASVVRGLAYYTGIVFEGFDRKGKL 351
Query: 259 KALAGGGRYDNLSKIY-GKEYPAT----GGAVYLERL--LDILPKNVEKKDYFV--IDKT 309
+A+ GGGRYD L Y G ++PA G AV +E L D+LP+ ++ + V +DK
Sbjct: 352 RAICGGGRYDRLLSTYGGDDFPACGFGFGDAVIVELLKEKDLLPELGQEVENIVCALDKD 411
Query: 310 KKRLGLKLADVLREKGKKVGM 330
+ +A LR+KG+ V +
Sbjct: 412 LQGAAATVATALRDKGQTVDL 432
sp|Q02147|YHI3_LACLA HYPOTHETICAL 38.0 KD PROTEIN IN HISC-HISG INTERGENIC REGION (ORF3)
>gi|477937|pir||C45734 histidyl-tRNA synthetase homolog
- Lactococcus lactis subsp. lactis >gi|2565140 (U92974)
unknown [Lactococcus lactis]
Length = 328
Score = 74.9 bits (181), Expect = 1e-12
Identities = 73/314 (23%), Positives = 133/314 (42%), Gaps = 27/314 (8%)
Query: 13 SLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEG---SKEAITFKDSYT 69
+L + +++I + K+F Y + FE + G ++E + ++
Sbjct: 16 TLNQVKSLRQIEGRLRKLFSLKNYQEVMPPSFEYTQLYTALESNGKTFNQEKMFQFINHE 75
Query: 70 KESFGLRLDFTTQVVRTISHLRNVKLPERVYYFGKVFSLDRR----GFEKLQTGVELIGE 125
+S LR DFT +VR S +++ R YFGK+F ++R E Q G+EL GE
Sbjct: 76 GQSITLRYDFTLPLVRLYSQIKD-STSARYSYFGKIFRKEKRHKGRSTENYQIGIELFGE 134
Query: 126 KSILADFEVIQVLTEFLKSLGLKDLKVILSHAGIVRK---VADEREEL------------ 170
+ ++ E++ + + ++ LGL + A ++ +AD EL
Sbjct: 135 SADKSELEILSLALQVIEQLGLNKTVFEIGSAKFFQRLCHLADGSTELLTELLLKKDLSG 194
Query: 171 LRAFSERN--LEALKEVLGNDIKFFVEVSKNPEVLNFXXXXXXXXXXXXXXXXXXXXXXF 228
L AF E+N + L+E+L +I E+S+ ++
Sbjct: 195 LNAFIEKNNFSKELRELL-KEIFITNELSRLENLVTNTKDDVLISSFDQLKEFSEKLSMI 253
Query: 229 GIDYAYDLGEVRNFPYYTGVIFEIYELKSGKALAGGGRYDNLSKIYGKEYPATGGAVYLE 288
DLG V YYT ++F+ Y + + + GGRYD L + +E A G +++
Sbjct: 254 K-PIIIDLGMVPKMDYYTDLMFKAYSSAANQPILSGGRYDQLLSNFQEEAVAIGFCCHMD 312
Query: 289 RLLDILPKNVEKKD 302
+L L + ++D
Sbjct: 313 TILKALERQELEED 326
sp|P30053|SYH_STREQ HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
Length = 426
Score = 72.2 bits (174), Expect = 7e-12
Identities = 83/299 (27%), Positives = 124/299 (40%), Gaps = 36/299 (12%)
Query: 27 AVKIFEAFGYDYINLSHFEPYEFQELTFGEGS----KEAITFKDSYTKESFGLRLDFTTQ 82
A + F+ + Y I FE YE + G+ + KE F D + LR + T
Sbjct: 27 ARETFKQYHYGEIRTPMFEHYEVISRSVGDTTDIVTKEMYDFYDKGDRH-ITLRPEGTAP 85
Query: 83 VVRTISHLRN------VKLPERVYYFGKVFSLDRRGFEKL----QTGVELIGEKSILADF 132
VVR S++ N V+ P ++YY G +F +R +L Q GVE G + D
Sbjct: 86 VVR--SYVENKLFAPEVQKPVKLYYIGSMFRYERPQAGRLREFHQIGVECFGSANPATDV 143
Query: 133 EVIQVLTEFLKSLGLKDLKVILSHAGIVRKVADEREEL----------LRAFSERNLE-- 180
E I + + LG+K + + L+ G A R+ L L S+R L+
Sbjct: 144 ETIAMAYHLFERLGIKGVTLHLNSLGNAASRAAYRQALIDYLSPMRDTLSKDSQRRLDEN 203
Query: 181 ALKEVLGNDIKFFVEVSKNPEVLNFXXXXXXXXXXXXXXXXXXXXXXFGIDYAYDLGEVR 240
L+ + + + + V+ P +L++ I Y D VR
Sbjct: 204 PLRVLDSKEKEDKIAVANAPSILDY----QDEESQAHFDAVRSMLEALAIPYVIDTNMVR 259
Query: 241 NFPYYTGVIFE-IYEL-KSGKALAGGGRYDNLSKIY-GKEYPATGGAVYLERLLDILPK 296
YY IFE I E+ +S + GGRYD L + + G P G + LERLL IL K
Sbjct: 260 GLDYYNHTIFEFITEVDQSELTICAGGRYDGLVEYFGGPATPGFGFGLGLERLLLILDK 318
pir||S30232 histidine--tRNA ligase (EC 6.1.1.21) - Streptococcus "equisimilis"
(fragment) >gi|1333826|emb|CAA78919| (Z17214)
histidine--tRNA ligase [Streptococcus equisimilis]
Length = 407
Score = 72.2 bits (174), Expect = 7e-12
Identities = 83/299 (27%), Positives = 124/299 (40%), Gaps = 36/299 (12%)
Query: 27 AVKIFEAFGYDYINLSHFEPYEFQELTFGEGS----KEAITFKDSYTKESFGLRLDFTTQ 82
A + F+ + Y I FE YE + G+ + KE F D + LR + T
Sbjct: 8 ARETFKQYHYGEIRTPMFEHYEVISRSVGDTTDIVTKEMYDFYDKGDRH-ITLRPEGTAP 66
Query: 83 VVRTISHLRN------VKLPERVYYFGKVFSLDRRGFEKL----QTGVELIGEKSILADF 132
VVR S++ N V+ P ++YY G +F +R +L Q GVE G + D
Sbjct: 67 VVR--SYVENKLFAPEVQKPVKLYYIGSMFRYERPQAGRLREFHQIGVECFGSANPATDV 124
Query: 133 EVIQVLTEFLKSLGLKDLKVILSHAGIVRKVADEREEL----------LRAFSERNLE-- 180
E I + + LG+K + + L+ G A R+ L L S+R L+
Sbjct: 125 ETIAMAYHLFERLGIKGVTLHLNSLGNAASRAAYRQALIDYLSPMRDTLSKDSQRRLDEN 184
Query: 181 ALKEVLGNDIKFFVEVSKNPEVLNFXXXXXXXXXXXXXXXXXXXXXXFGIDYAYDLGEVR 240
L+ + + + + V+ P +L++ I Y D VR
Sbjct: 185 PLRVLDSKEKEDKIAVANAPSILDY----QDEESQAHFDAVRSMLEALAIPYVIDTNMVR 240
Query: 241 NFPYYTGVIFE-IYEL-KSGKALAGGGRYDNLSKIY-GKEYPATGGAVYLERLLDILPK 296
YY IFE I E+ +S + GGRYD L + + G P G + LERLL IL K
Sbjct: 241 GLDYYNHTIFEFITEVDQSELTICAGGRYDGLVEYFGGPATPGFGFGLGLERLLLILDK 299
sp|P56194|SYH_THETH HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|2392059|pdb|1ADJ|A Chain A, Histidyl-Trna Synthetase
In Complex With Histidine >gi|2392061|pdb|1ADJ|B Chain
B, Histidyl-Trna Synthetase In Complex With Histidine
>gi|2392063|pdb|1ADJ|C Chain C, Histidyl-Trna Synthetase
In Complex With Histidine >gi|2392065|pdb|1ADJ|D Chain
D, Histidyl-Trna Synthetase In Complex With Histidine
>gi|2392067|pdb|1ADY|A Chain A, Histidyl-Trna Synthetase
In Complex With Histidyl-Adenylate
>gi|2392068|pdb|1ADY|B Chain B, Histidyl-Trna Synthetase
In Complex With Histidyl-Adenylate
>gi|2392069|pdb|1ADY|C Chain C, Histidyl-Trna Synthetase
In Complex With Histidyl-Adenylate
>gi|2392070|pdb|1ADY|D Chain D, Histidyl-Trna Synthetase
In Complex With Histidyl-Adenylate
Length = 421
Score = 71.0 bits (171), Expect = 2e-11
Identities = 87/318 (27%), Positives = 129/318 (40%), Gaps = 34/318 (10%)
Query: 1 MKTSLISGERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGS-- 58
M + G + +E + + I A K+ EA G + FE + E G +
Sbjct: 1 MTARAVRGTKDLFGKELRMHQRIVATARKVLEAAGALELVTPIFEETQVFEKGVGAATDI 60
Query: 59 --KEAITFKDSYTKESFGLRLDFTTQVVRT-ISHLRNV-KLPERVYYFGKVFSLDR---- 110
KE TF+D + S LR + T +VR + H V P R++ G +F +R
Sbjct: 61 VRKEMFTFQDRGGR-SLTLRPEGTAAMVRAYLEHGMKVWPQPVRLWMAGPMFRAERPQKG 119
Query: 111 RGFEKLQTGVELIGEKSILADFEVIQVLTEFLKSLGLKDLKVILSHAG----------IV 160
R + Q E +G ++ + D E + +L E LK LGL+ LKV LS G +
Sbjct: 120 RYRQFHQVNYEALGSENPILDAEAVVLLYECLKELGLRRLKVKLSSVGDPEDRARYNAYL 179
Query: 161 RKVADEREELLRAFSERNLE-----ALKEVLGNDIKFFVEVSKNPEVLNFXXXXXXXXXX 215
R+V E L S+ LE L D E+ P +L+F
Sbjct: 180 REVLSPHREALSEDSKERLEENPMRILDSKSERDQALLKELGVRP-MLDF----LGEEAR 234
Query: 216 XXXXXXXXXXXXFGIDYAYDLGEVRNFPYYTGVIFEIY--ELKSGKALAGGGRYDNLSKI 273
+ Y + VR YY FE++ E+ + AL GGGRYD LS++
Sbjct: 235 AHLKEVERHLERLSVPYELEPALVRGLDYYVRTAFEVHHEEIGAQSALGGGGRYDGLSEL 294
Query: 274 Y-GKEYPATGGAVYLERL 290
G P G A +ER+
Sbjct: 295 LGGPRVPGVGFAFGVERV 312
sp|P12081|SYH_HUMAN HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|2144560|pir||SYHUHT histidine--tRNA ligase (EC
6.1.1.21) - human >gi|32460|emb|CAA77607| (Z11518)
histidyl-tRNA synthetase [Homo sapiens]
Length = 509
Score = 68.3 bits (164), Expect = 1e-10
Identities = 99/421 (23%), Positives = 165/421 (38%), Gaps = 75/421 (17%)
Query: 8 GERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGSKEAITFKDS 67
G R YS + + +++F ++ F+ G + I+ FE E +GE SK KD
Sbjct: 61 GTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLIYDLKDQ 120
Query: 68 YTKESFGLRLDFTTQVVR--TISHLRNVKLPERVYYFGKVFSLDR------RGFEKLQTG 119
E LR D T R ++ L N+K Y+ KV+ D R E Q
Sbjct: 121 -GGELLSLRYDLTVPFARYLAMNKLTNIKR----YHIAKVYRRDNPAMTRGRYREFYQCD 175
Query: 120 VELIGE-KSILADFEVIQVLTEFLKSLGLKDLKV------ILSHAGIVRKVADEREELLR 172
++ G ++ D E ++++ E L SL + D V IL + V+D + +
Sbjct: 176 FDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSKFRTIC 235
Query: 173 AFSERNLEALKEVLGNDIKFFVEVSKN--PEVLN-----------------------FXX 207
+ +++ L +V ++K + K PEV +
Sbjct: 236 S----SVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQ 291
Query: 208 XXXXXXXXXXXXXXXXXXXXFGID--YAYDLGEVRNFPYYTGVIFEIYELKSGK------ 259
FGID ++DL R YYTGVI+E L++
Sbjct: 292 NKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEP 351
Query: 260 ----ALAGGGRYDNLSKIY---GKEYPATGGAVYLERLLDILPKNVE---------KKDY 303
++A GGRYD L ++ G++ P G ++ +ER+ I+ + +E +
Sbjct: 352 LGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTETQV 411
Query: 304 FVIDKTKKRL--GLKLADVLREKGKKVGMEIVKERGLEHSLIYAFDKGFKEVLLVEDEIV 361
V KK L LKL L + G K + K L + L Y + G V ++ ++ +
Sbjct: 412 LVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQEL 471
Query: 362 K 362
K
Sbjct: 472 K 472
sp|O83647|SYH_TREPA HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|3322940 (AE001239) histidyl-tRNA synthetase (hisS)
[Treponema pallidum]
Length = 442
Score = 67.9 bits (163), Expect = 1e-10
Identities = 58/273 (21%), Positives = 116/273 (42%), Gaps = 39/273 (14%)
Query: 55 GEGSKEAITFKDSYTKESFGLRLDFTTQVVRTIS-HLRNVKLPERVYYFGKVFSLDR--- 110
G+ K+ F D ++ LR D T + R ++ H + P + Y+F KV+ ++
Sbjct: 62 GDTEKQMFRFVDKGGRD-VALRFDLTVPLARFVATHYARLYFPFKRYHFAKVWRGEKPQM 120
Query: 111 -RGFEKLQTGVELIGEKSILADFEVIQVLTEFLKSLGLKDLKVILSHAGIVRK------V 163
R E Q +++G S+ ADFE+++ + L G + +++ ++H G+ + +
Sbjct: 121 GRYREFTQCDFDIVGSDSVCADFEILKSIRHMLYMAGAEHIRIHVAHRGLFDRFLRALSL 180
Query: 164 ADEREELLRAFSERNLEALKEVLGNDIKFFVEVSKNPEVLNFXXXXXXXXXXXXXXXXXX 223
+D+ E +LR +R + VL ++ + + +++ +
Sbjct: 181 SDQAEHILRIIDKR-AKMAPHVLTAQLESLCDPVRVQKIMTYVSAGEVDGVAPSFEHTLS 239
Query: 224 XXXXF--GI---------------------DYAYDLGEVRNFPYYTGVIFEIY--ELKSG 258
G+ Y +D R F YYTG++ E + +L
Sbjct: 240 AIETLTGGVSEESTRLRKIYELLCAVNIQSSYVFDPSITRGFDYYTGMVCETFLTQLPHI 299
Query: 259 KALAGGGRYDNLSKIYGKE-YPATGGAVYLERL 290
++ GGRYD+L+ +Y K+ G ++ L+RL
Sbjct: 300 GSVCSGGRYDHLTALYMKDAVSGVGASIGLDRL 332
ref|NP_032240.1|| histidyl tRNA synthetase >gi|2501005|sp|Q61035|SYH_MOUSE
HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE)
(HISRS) >gi|2137380|pir||JC5223 histidine--tRNA ligase
(EC 6.1.1.21) - mouse >gi|1209683 (U39473) histidyl-tRNA
synthetase [Mus musculus]
Length = 509
Score = 67.5 bits (162), Expect = 2e-10
Identities = 97/417 (23%), Positives = 168/417 (40%), Gaps = 67/417 (16%)
Query: 8 GERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGSKEAITFKDS 67
G R YS + + +++F ++ F+ G + I+ FE E +GE SK KD
Sbjct: 61 GTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKETLTGKYGEDSKLIYDLKDQ 120
Query: 68 YTKESFGLRLDFTTQVVR--TISHLRNVKLPERVYYFGKVFSLDR------RGFEKLQTG 119
E LR D T R ++ L N+K Y+ KV+ D R E Q
Sbjct: 121 -GGELLSLRYDLTVPFARYLAMNKLTNIKR----YHIAKVYRRDNPAMTRGRYREFYQCD 175
Query: 120 VELIGE-KSILADFEVIQVLTEFLKSL----------------------GLKDLK--VIL 154
++ G+ ++ D E ++++ E L SL G+ D K I
Sbjct: 176 FDIAGQFDPMIPDAECLKIMCEILSSLQIGNFLVKVNDRRILDGMFAVCGVPDSKFRTIC 235
Query: 155 SHAGIVRKVADEREELLRAFSERNLEALKEVLGNDIKFFVEVSKNPEVLN---FXXXXXX 211
S + KV+ E + + + + +G+ ++ VS ++L
Sbjct: 236 SSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQA 295
Query: 212 XXXXXXXXXXXXXXXXFGID--YAYDLGEVRNFPYYTGVIFEIYEL----KSGK------ 259
FGID ++DL R YYTGVI+E L ++G+
Sbjct: 296 VEGLGDLKLLFEYLILFGIDDKISFDLSLARGLDYYTGVIYEAVLLQMPTQAGEEPLGVG 355
Query: 260 ALAGGGRYDNLSKIY---GKEYPATGGAVYLERLLDILPKNVEKKD--------YFVIDK 308
++A GGRYD L ++ G++ P G ++ +ER+ I+ + +E + ++
Sbjct: 356 SIAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEASEEKVRTTETQVLVAS 415
Query: 309 TKKRL---GLKLADVLREKGKKVGMEIVKERGLEHSLIYAFDKGFKEVLLVEDEIVK 362
+K+L LKL L + G K + K L + L Y + G V ++ ++ +K
Sbjct: 416 AQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELK 472
sp|O51160|SYH_BORBU HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|2688030 (AE001125) histidyl-tRNA synthetase (hisS)
[Borrelia burgdorferi]
Length = 457
Score = 66.0 bits (158), Expect = 5e-10
Identities = 78/354 (22%), Positives = 142/354 (40%), Gaps = 49/354 (13%)
Query: 1 MKTSLISGERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFG-EGSK 59
M + G + Y ++S + I ++ + ++ +D I+ E + G E K
Sbjct: 2 MDIKTLKGFKDYLPKDSLIRIHIVRQIFSVLNSYNFDLIDTPVLEYSDLLLKKSGDETEK 61
Query: 60 EAITFKDSYTKESFGLRLDFTTQVVRTIS-HLRNVKLPERVYYFGKVF----SLDRRGFE 114
+ FKD+ ++ +R D T R ++ ++ +KLP R FGKVF S R E
Sbjct: 62 QIYRFKDNGGRD-VSMRFDLTVPFARFVATNISALKLPFRRSQFGKVFRGENSQKGRYRE 120
Query: 115 KLQTGVELIGEKSILADFEVIQV-----------------------------LTEFLKSL 145
+Q +++GE + D E++ V L F + L
Sbjct: 121 FMQFDFDIVGEDTFRGDAEILSVVYYGLEEIFLNFIEGINKKFIIHYSHIGILNSFFEKL 180
Query: 146 GLKDLKV-ILSHAGIVRKVADER--EELLRAFSERNLEALKEVLGNDIKFFVEVSKNPEV 202
GLK+ + IL + + K+ ++ E LL + ++++ ++ F ++ +
Sbjct: 181 GLKEKSIFILRNIDKIDKIGIDKVKEALLLEIEKEAVDSILSLVSLQGTFKDKIQALKSI 240
Query: 203 LNFXXXXXXXXXXXXXXXXXXXXXXFGIDYAYDLGEVRNFPYYTGVIFEIYELKSGK-AL 261
L F ++ R YYTG++FE S ++
Sbjct: 241 LGDNESIKRVEDVFQHLSLLKIQDSFNLNLKIS----RGLDYYTGIVFESEVFGSNMGSV 296
Query: 262 AGGGRYDNLSKIYG---KEYPATGGAVYLERLLDILPKNVEKKDYFVIDKTKKR 312
GGRYDNL + ++ GG+ ++R+ DI+ ++EK Y I TK R
Sbjct: 297 CSGGRYDNLVSSFSNSIQKISGVGGSFGVDRIKDII--DLEKFSYIKIFVTKAR 348
pdb|1HTT|A Chain A, Histidyl-Trna Synthetase >gi|1942449|pdb|1HTT|B Chain B,
Histidyl-Trna Synthetase >gi|1942450|pdb|1HTT|C Chain C,
Histidyl-Trna Synthetase >gi|1942451|pdb|1HTT|D Chain D,
Histidyl-Trna Synthetase
Length = 423
Score = 66.0 bits (158), Expect = 5e-10
Identities = 86/312 (27%), Positives = 132/312 (41%), Gaps = 32/312 (10%)
Query: 6 ISGERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGS----KEA 61
I G Y E+ + + I + ++GY I L E + GE + KE
Sbjct: 7 IRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLPIVEQTPLFKRAIGEVTDVVEKEM 66
Query: 62 ITFKDSYTKESFGLRLDFTTQVVRT-ISHLRNVKLPERVYYFGKVFSLDR------RGFE 114
TF+D +S LR + T VR I H +R++Y G +F +R R F
Sbjct: 67 YTFEDR-NGDSLTLRPEGTAGCVRAGIEHGLLYNQEQRLWYIGPMFRHERPQKGRYRQFH 125
Query: 115 KLQTGVELIGEKSILADFEVIQVLTEFLKSLGLKD-LKVILSHAGIVRKVADEREELLRA 173
+L G E+ G + D E+I + + ++LG+ + + + L+ G + A+ R+ L+ A
Sbjct: 126 QL--GCEVFGLQGPDIDAELIMLTARWWRALGISEHVTLELNSIGSLEARANYRDALV-A 182
Query: 174 FSERNLEALKE-----VLGNDIKFFVEVSKNPEVLNFXXXXXXXXXXXXXXXXXXXXX-- 226
F E++ E L E + N ++ V SKNPEV
Sbjct: 183 FLEQHKEKLDEDCKRRMYTNPLR--VLDSKNPEVQALLNDAPALGDYLDEESREHFAGLC 240
Query: 227 ----XFGIDYAYDLGEVRNFPYYTGVIFE--IYELKSGKALAGGGRYDNL-SKIYGKEYP 279
GI Y + VR YY +FE L S + GGRYD L ++ G+ P
Sbjct: 241 KLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSLGSQGTVCAGGRYDGLVEQLGGRATP 300
Query: 280 ATGGAVYLERLL 291
A G A+ LERL+
Sbjct: 301 AVGFAMGLERLV 312
sp|P04804|SYH_ECOLI HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|68569|pir||SYECH histidine--tRNA ligase (EC
6.1.1.21) - Escherichia coli >gi|2781066|pdb|1KMM|A
Chain A, Histidyl-Trna Synthetase Complexed With
Histidyl-Adenylate >gi|2781067|pdb|1KMM|B Chain B,
Histidyl-Trna Synthetase Complexed With
Histidyl-Adenylate >gi|2781068|pdb|1KMM|C Chain C,
Histidyl-Trna Synthetase Complexed With
Histidyl-Adenylate >gi|2781069|pdb|1KMM|D Chain D,
Histidyl-Trna Synthetase Complexed With
Histidyl-Adenylate >gi|2781070|pdb|1KMN|A Chain A,
Histidyl-Trna Synthetase Complexed With Histidinol And
Atp >gi|2781071|pdb|1KMN|B Chain B, Histidyl-Trna
Synthetase Complexed With Histidinol And Atp
>gi|2781072|pdb|1KMN|C Chain C, Histidyl-Trna Synthetase
Complexed With Histidinol And Atp >gi|2781073|pdb|1KMN|D
Chain D, Histidyl-Trna Synthetase Complexed With
Histidinol And Atp >gi|146372 (M11843) histidine-tRNA
synthetase [Escherichia coli] >gi|1788861 (AE000337)
histidine tRNA synthetase [Escherichia coli]
>gi|1805574|dbj|BAA16401| (D90880) histidine--tRNA
ligase (EC 6.1.1.21) [Escherichia coli]
Length = 424
Score = 66.0 bits (158), Expect = 5e-10
Identities = 86/312 (27%), Positives = 132/312 (41%), Gaps = 32/312 (10%)
Query: 6 ISGERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGS----KEA 61
I G Y E+ + + I + ++GY I L E + GE + KE
Sbjct: 8 IRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLPIVEQTPLFKRAIGEVTDVVEKEM 67
Query: 62 ITFKDSYTKESFGLRLDFTTQVVRT-ISHLRNVKLPERVYYFGKVFSLDR------RGFE 114
TF+D +S LR + T VR I H +R++Y G +F +R R F
Sbjct: 68 YTFEDR-NGDSLTLRPEGTAGCVRAGIEHGLLYNQEQRLWYIGPMFRHERPQKGRYRQFH 126
Query: 115 KLQTGVELIGEKSILADFEVIQVLTEFLKSLGLKD-LKVILSHAGIVRKVADEREELLRA 173
+L G E+ G + D E+I + + ++LG+ + + + L+ G + A+ R+ L+ A
Sbjct: 127 QL--GCEVFGLQGPDIDAELIMLTARWWRALGISEHVTLELNSIGSLEARANYRDALV-A 183
Query: 174 FSERNLEALKE-----VLGNDIKFFVEVSKNPEVLNFXXXXXXXXXXXXXXXXXXXXX-- 226
F E++ E L E + N ++ V SKNPEV
Sbjct: 184 FLEQHKEKLDEDCKRRMYTNPLR--VLDSKNPEVQALLNDAPALGDYLDEESREHFAGLC 241
Query: 227 ----XFGIDYAYDLGEVRNFPYYTGVIFE--IYELKSGKALAGGGRYDNL-SKIYGKEYP 279
GI Y + VR YY +FE L S + GGRYD L ++ G+ P
Sbjct: 242 KLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSLGSQGTVCAGGRYDGLVEQLGGRATP 301
Query: 280 ATGGAVYLERLL 291
A G A+ LERL+
Sbjct: 302 AVGFAMGLERLV 313
gi|2896164 (AF047040) histidyl-tRNA synthetase; aminoacyl-tRNA synthetase
[Salmonella typhimurium]
Length = 424
Score = 65.6 bits (157), Expect = 7e-10
Identities = 87/312 (27%), Positives = 134/312 (42%), Gaps = 32/312 (10%)
Query: 6 ISGERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGS----KEA 61
I G Y E+ + + I + ++GY I L E + GE + KE
Sbjct: 8 IRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLPIVEQTPLFKRAIGEVTDVVEKEM 67
Query: 62 ITFKDSYTKESFGLRLDFTTQVVRT-ISHLRNVKLPERVYYFGKVFSLDR------RGFE 114
TF+D +S LR + T VR I H +R++Y G +F +R R F
Sbjct: 68 YTFEDR-NGDSLTLRPEGTAGCVRAGIEHGLLYNQEQRLWYIGPMFRHERPQKGRYRQFH 126
Query: 115 KLQTGVELIGEKSILADFEVIQVLTEFLKSLGLKD-LKVILSHAGIVRKVADEREELLRA 173
+L G E+ G + D E+I + + ++LG+ + + + L+ G + A+ R+ L+ A
Sbjct: 127 QL--GAEVFGLQGPDIDAELIMLTARWWRALGIAEHVSLELNSIGSLEARANYRDALV-A 183
Query: 174 FSERNLEALKE-----VLGNDIKFFVEVSKNPEV---LNFXXXXXXXXXXXXXXXXX--- 222
F E++ E L E + N ++ V SKNP+V LN
Sbjct: 184 FLEQHQETLDEDCKRRMYTNPLR--VLDSKNPDVQALLNDAPALGDYLDDDSREHFAGLC 241
Query: 223 XXXXXFGIDYAYDLGEVRNFPYYTGVIFE--IYELKSGKALAGGGRYDNL-SKIYGKEYP 279
GI Y + VR YY +FE L S + GGRYD L ++ G+ P
Sbjct: 242 KLLDAAGIAYTVNQRLVRGLDYYNRTVFEWVTNSLGSQGTVCAGGRYDGLVEQLGGRATP 301
Query: 280 ATGGAVYLERLL 291
A G A+ LERL+
Sbjct: 302 AVGFAMGLERLV 313
gb|AAF10919.1|AE001981_4 (AE001981) histidyl-tRNA synthetase [Deinococcus radiodurans]
Length = 427
Score = 64.8 bits (155), Expect = 1e-09
Identities = 77/303 (25%), Positives = 128/303 (41%), Gaps = 45/303 (14%)
Query: 23 IFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGSKEAITFKDSYTKESFG------LR 76
+ +A ++ E G I+ FE E + G G I K+ +T FG LR
Sbjct: 32 LIAQAREVLERAGAQRIDTPMFEEAELVKR--GVGGSTDIVRKEMFTVYYFGDHGGYVLR 89
Query: 77 LDFTTQVVRTI--SHLRNVKLPERVYYFGKVFSLDR----RGFEKLQTGVELIGEKSILA 130
+ T +VR + L+ + P +++ G +F +R R + Q E++G L
Sbjct: 90 PEGTASIVRAYLENGLKQLPAPLKLWTSGPMFRAERHQKGRYRQFTQVDYEVLGSADALV 149
Query: 131 DFEVIQVLTEFLKSLGLKDLKVILSHAGI------------------VRKVADEREELLR 172
D E I ++ ++ LGL+ ++V L G + +++D+ ++ L
Sbjct: 150 DAEAIALMVAVVQKLGLRGVRVKLGSIGDPEDRERYNAYLRDLFSPHLGRLSDDSKDRL- 208
Query: 173 AFSERN-LEALKEVLGNDIKFFVEVSKNPEVLNFXXXXXXXXXXXXXXXXXXXXXXFGID 231
ERN + L +D + E+S P +L+F +G+
Sbjct: 209 ---ERNPMRILDSKSASDQELLAELSVRP-MLDFLGEGAAADFRQVQAYLSD----WGVP 260
Query: 232 YAYDLGEVRNFPYYTGVIFEI-YE-LKSGKALAGGGRYDNLS-KIYGKEYPATGGAVYLE 288
+ D VR YY +E+ YE + + AL GGGRYD LS ++ G P G A +E
Sbjct: 261 FDLDPSIVRGLDYYRRTAWELHYEGIGAKSALGGGGRYDGLSEQLGGGSVPGIGWAFGVE 320
Query: 289 RLL 291
RLL
Sbjct: 321 RLL 323
sp|P93422|SYH_ORYSA HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|1841704|emb|CAB06653| (Z85984) histidyl tRNA
Synthetase [Oryza sativa]
Length = 494
Score = 64.8 bits (155), Expect = 1e-09
Identities = 95/406 (23%), Positives = 158/406 (38%), Gaps = 62/406 (15%)
Query: 8 GERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGSKEAITFKDS 67
G R + E+ + + F +F+ G ++ FE E +GE SK D
Sbjct: 64 GTRDFGKEQMAIREHAFSIITGVFKMHGAVSLDTPVFELRETLMGKYGEDSKLIYDLADQ 123
Query: 68 YTKESFGLRLDFTTQVVRTISHLRNVKLPERVYYFGKVFSLDR----RGFEKLQTGVELI 123
E LR D T R ++ + N+ +R Y KV+ D R E Q ++
Sbjct: 124 -GGELCSLRYDLTVPFARYVA-MNNISSLKR-YQIAKVYRRDNPSKGRYREFYQCDFDIA 180
Query: 124 GE-KSILADFEVIQVLTEFLKSLGLKDLKVILSHAGIVRKVADEREEL------------ 170
G +++ DFEVI+VLTE L L + ++ L+H RK+ D E+
Sbjct: 181 GVYETMEPDFEVIKVLTELLDQLDIGTYEIKLNH----RKLLDGMLEICGVPPEKFRTVC 236
Query: 171 --LRAFSERNLEALKEVL---------------------GNDIKFFVEVSKNPEVLNFXX 207
+ ++ E +K+ L G ++ +E+ K
Sbjct: 237 SSIDKLDKQTFEQVKKELVDEKGISNETADKIGDLVKTRGPPLEVLLELRKEGSKFMGNA 296
Query: 208 XXXXXXXXXXXXXXXXXXXXFGIDYAYDLGEVRNFPYYTGVIFEIYELKSGK--ALAGGG 265
+DL R YYTGVI+E + + ++A GG
Sbjct: 297 GSVTALNELEILFKALDKANAIGKIVFDLSLARGLDYYTGVIYEAVFKGTTQVGSIAAGG 356
Query: 266 RYDNLSKIY-GKEYPATGGAVYLERLLDILPKNVEKKDYFVIDKTKKR---------LGL 315
RYDNL ++ GK+ PA G ++ +ER+ I+ + +K+ +T+ L
Sbjct: 357 RYDNLVGMFSGKQVPAVGVSLGIERVFAIMEQQEMEKNQIRATETEVLVSIIGKDLILAA 416
Query: 316 KLADVLREKGKKVGMEIVKERGLEHSLIYAFDKGFKEVLLV-EDEI 360
+L L G K ++ +++ L YA G ++LV E EI
Sbjct: 417 ELVSELWNAGIKAEFKLTTR--IQNHLKYATQSGIPWMVLVGESEI 460
ref|NP_015358.1|HTS1| cytoplasmic and mitochondrial histidine tRNA synthetases; Hts1p
>gi|2507433|sp|P07263|SYH_YEAST HISTIDYL-TRNA
SYNTHETASE, MITOCHONDRIAL PRECURSOR (HISTIDINE--TRNA
LIGASE) (HISRS) >gi|2144561|pir||SYBYHM histidine--tRNA
ligase (EC 6.1.1.21) precursor, mitochondrial - yeast
(Saccharomyces cerevisiae) >gi|809598|emb|CAA89287|
(Z49274) Hts1p [Saccharomyces cerevisiae]
>gi|1314107|emb|CAA94983| (Z71255) Hts1p [Saccharomyces
cerevisiae]
Length = 546
Score = 64.4 bits (154), Expect = 1e-09
Identities = 82/344 (23%), Positives = 137/344 (38%), Gaps = 71/344 (20%)
Query: 23 IFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGSKEAITFKDSYTKESFGLRLDFTTQ 82
IF +F+ G I+ FE E +GE SK +D E LR D T
Sbjct: 75 IFSTLSGLFKKHGGVTIDTPVFELREILAGKYGEDSKLIYNLEDQ-GGELCSLRYDLTVP 133
Query: 83 VVRTISHLRNVKLPERVYYFGKVFSLDRRGFEK------LQTGVELIGE-KSILADFEVI 135
R ++ + N++ +R Y+ KV+ D+ K Q ++ G +S++ D E +
Sbjct: 134 FARYVA-MNNIQSIKR-YHIAKVYRRDQPAMTKGRMREFYQCDFDVAGTFESMVPDSECL 191
Query: 136 QVLTEFLKSLGLKDLKVILSHAGI---VRKVADEREELLRAFS-------ERNLEALK-- 183
+L E L SLG+KD K+ L+H I + ++A ++E +R S + EA+K
Sbjct: 192 SILVEGLTSLGIKDFKIKLNHRKILDGIFQIAGVKDEDVRKISSAVDKLDKSPWEAVKKE 251
Query: 184 ------------EVLGNDIKFFVEVSKNPEVL----NFXXXXXXXXXXXXXXXXXXXXXX 227
+ +G +K + + VL N
Sbjct: 252 MTEEKGQSEETADKIGEYVKLNGSLKEIHAVLSADANITSNEKAKQGLDDIATLMKYTEA 311
Query: 228 FGID--YAYDLGEVRNFPYYTGVIFEIY-----------ELKSGK-------------AL 261
F ID ++DL R YYTG+I+E+ ELK ++
Sbjct: 312 FDIDSFISFDLSLARGLDYYTGLIYEVVTSASAPPENASELKKKAKSAEDASEFVGVGSI 371
Query: 262 AGGGRYDNLSKIYGK-------EYPATGGAVYLERLLDILPKNV 298
A GGRYDNL ++ + + P G + +ER+ ++ + +
Sbjct: 372 AAGGRYDNLVNMFSEASGKKSTQIPCVGISFGVERIFSLIKQRI 415
pir||SYBYHC histidine--tRNA ligase (EC 6.1.1.21), cytosolic - yeast
(Saccharomyces cerevisiae) >gi|171733 (M14048)
histidine-tRNA synthetase cytoplasmic [Saccharomyces
cerevisiae]
Length = 526
Score = 64.4 bits (154), Expect = 1e-09
Identities = 82/344 (23%), Positives = 137/344 (38%), Gaps = 71/344 (20%)
Query: 23 IFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGSKEAITFKDSYTKESFGLRLDFTTQ 82
IF +F+ G I+ FE E +GE SK +D E LR D T
Sbjct: 55 IFSTLSGLFKKHGGVTIDTPVFELREILAGKYGEDSKLIYNLEDQ-GGELCSLRYDLTVP 113
Query: 83 VVRTISHLRNVKLPERVYYFGKVFSLDRRGFEK------LQTGVELIGE-KSILADFEVI 135
R ++ + N++ +R Y+ KV+ D+ K Q ++ G +S++ D E +
Sbjct: 114 FARYVA-MNNIQSIKR-YHIAKVYRRDQPAMTKGRMREFYQCDFDVAGTFESMVPDSECL 171
Query: 136 QVLTEFLKSLGLKDLKVILSHAGI---VRKVADEREELLRAFS-------ERNLEALK-- 183
+L E L SLG+KD K+ L+H I + ++A ++E +R S + EA+K
Sbjct: 172 SILVEGLTSLGIKDFKIKLNHRKILDGIFQIAGVKDEDVRKISSAVDKLDKSPWEAVKKE 231
Query: 184 ------------EVLGNDIKFFVEVSKNPEVL----NFXXXXXXXXXXXXXXXXXXXXXX 227
+ +G +K + + VL N
Sbjct: 232 MTEEKGQSEETADKIGEYVKLNGSLKEIHAVLSADANITSNEKAKQGLDDIATLMKYTEA 291
Query: 228 FGID--YAYDLGEVRNFPYYTGVIFEIY-----------ELKSGK-------------AL 261
F ID ++DL R YYTG+I+E+ ELK ++
Sbjct: 292 FDIDSFISFDLSLARGLDYYTGLIYEVVTSASAPPENASELKKKAKSAEDASEFVGVGSI 351
Query: 262 AGGGRYDNLSKIYGK-------EYPATGGAVYLERLLDILPKNV 298
A GGRYDNL ++ + + P G + +ER+ ++ + +
Sbjct: 352 AAGGRYDNLVNMFSEASGKKSTQIPCVGISFGVERIFSLIKQRI 395
gi|171732 (M14048) histidine-tRNA synthetase mitochondrial [Saccharomyces
cerevisiae]
Length = 546
Score = 64.4 bits (154), Expect = 1e-09
Identities = 82/344 (23%), Positives = 137/344 (38%), Gaps = 71/344 (20%)
Query: 23 IFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGSKEAITFKDSYTKESFGLRLDFTTQ 82
IF +F+ G I+ FE E +GE SK +D E LR D T
Sbjct: 75 IFSTLSGLFKKHGGVTIDTPVFELREILAGKYGEDSKLIYNLEDQ-GGELCSLRYDLTVP 133
Query: 83 VVRTISHLRNVKLPERVYYFGKVFSLDRRGFEK------LQTGVELIGE-KSILADFEVI 135
R ++ + N++ +R Y+ KV+ D+ K Q ++ G +S++ D E +
Sbjct: 134 FARYVA-MNNIQSIKR-YHIAKVYRRDQPAMTKGRMREFYQCDFDVAGTFESMVPDSECL 191
Query: 136 QVLTEFLKSLGLKDLKVILSHAGI---VRKVADEREELLRAFS-------ERNLEALK-- 183
+L E L SLG+KD K+ L+H I + ++A ++E +R S + EA+K
Sbjct: 192 SILVEGLTSLGIKDFKIKLNHRKILDGIFQIAGVKDEDVRKISSAVDKLDKSPWEAVKKE 251
Query: 184 ------------EVLGNDIKFFVEVSKNPEVL----NFXXXXXXXXXXXXXXXXXXXXXX 227
+ +G +K + + VL N
Sbjct: 252 MTEEKGQSEETADKIGEYVKLNGSLKEIHAVLSADANITSNEKAKQGLDDIATLMKYTEA 311
Query: 228 FGID--YAYDLGEVRNFPYYTGVIFEIY-----------ELKSGK-------------AL 261
F ID ++DL R YYTG+I+E+ ELK ++
Sbjct: 312 FDIDSFISFDLSLARGLDYYTGLIYEVVTSASAPPENASELKKKAKSAEDASEFVGVGSI 371
Query: 262 AGGGRYDNLSKIYGK-------EYPATGGAVYLERLLDILPKNV 298
A GGRYDNL ++ + + P G + +ER+ ++ + +
Sbjct: 372 AAGGRYDNLVNMFSEASGKKSTQIPCVGISFGVERIFSLIKQRI 415
gb|AAD18802| (AE001648) Histidyl tRNA Synthetase [Chlamydia pneumoniae]
Length = 430
Score = 63.2 bits (151), Expect = 3e-09
Identities = 82/286 (28%), Positives = 122/286 (41%), Gaps = 32/286 (11%)
Query: 34 FGYDYINLSHFEPYEFQELTFGEGS----KEAITFKDSYTKESFGLRLDFTTQVVRT-IS 88
+G+ I FE E L GE S KE +F D + S LR + T VVR+ +
Sbjct: 43 YGFCEIRTPIFEKSEVF-LHVGEESDVVKKEVYSFLDRKGR-SMTLRPEGTAAVVRSFLE 100
Query: 89 HLRNVKLPERVYYFGKVFSLDR----RGFEKLQTGVELIGEKSILADFEVIQVLTEFLKS 144
H + + + YY +F +R R + Q GVE IG + L D EV+ +L +F
Sbjct: 101 HGASHRSDNKFYYILPMFRYERQQAGRYRQHHQFGVEAIGVRHPLRDAEVLALLWDFYSR 160
Query: 145 LGLKDLKVILSHAGIVRKVADEREELLRAF---SERNLEALKEVLGNDIKFFVEVSKNPE 201
+GL+ +++ L+ G + +++LRA+ S L AL + + + SK PE
Sbjct: 161 VGLQHMQIQLNFLG-GSETRFRYDKVLRAYLKESMGELSALSQQRFSTNVLRILDSKEPE 219
Query: 202 ----------VLNFXXXXXXXXXXXXXXXXXXXXXXFGIDYAYDLGEVRNFPYYTGVIFE 251
+L++ I YA + VR YY+ ++FE
Sbjct: 220 DQEIIRQAPPILDY----VSDEDLKYFNEILDALRVLEIPYAINPRLVRGLDYYSDLVFE 275
Query: 252 IYEL--KSGKALAGGGRYDNL-SKIYGKEYPATGGAVYLERLLDIL 294
+ AL GGGRYD L S G PA G V LER + L
Sbjct: 276 ATTTFQEVSYALGGGGRYDGLISAFGGASLPACGFGVGLERAIQTL 321
gi|4103152 (AF020716) histidyl-tRNA synthetase [Triticum aestivum]
Length = 479
Score = 63.2 bits (151), Expect = 3e-09
Identities = 77/337 (22%), Positives = 132/337 (38%), Gaps = 42/337 (12%)
Query: 8 GERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGSKEAITFKDS 67
G R + E+ + + F +F+ G ++ FE E +GE SK D
Sbjct: 48 GTRDFGREQMAIRERAFSIITSVFKMHGGVALDTPVFELRETLMGKYGEDSKLVYDLADQ 107
Query: 68 YTKESFGLRLDFTTQVVRTISHLRNVKLPERVYYFGKVFSLDR----RGFEKLQTGVELI 123
E LR D T R ++ + N+ +R Y KV+ D R E Q ++
Sbjct: 108 -GGELCSLRYDLTVPFARYVA-MNNISAIKR-YQIAKVYRRDNPSKGRYREFYQCDFDIA 164
Query: 124 GE-KSILADFEVIQVLTEFLKSLGLKDLKVILSHAGIV--------------RKVADERE 168
G + + DFEV++VLTE L L + D ++ L+H ++ R V +
Sbjct: 165 GVYEPMEPDFEVVKVLTELLDKLDIGDYEIKLNHRKLLDGMLEICGVPAEKFRTVCSSID 224
Query: 169 ELLRAFSER-----------------NLEALKEVLGNDIKFFVEVSKNPEVLNFXXXXXX 211
+L + E N+ +L + G ++ +E+ K
Sbjct: 225 KLDKLTFEEVKKELVEEKGVSNETAENIGSLVKTRGPPLEVLLELRKEGSKFMENEGSVV 284
Query: 212 XXXXXXXXXXXXXXXXFGIDYAYDLGEVRNFPYYTGVIFEIY--ELKSGKALAGGGRYDN 269
++DL R YYTGVI+E + ++A GGRYD
Sbjct: 285 ALNELEILFKALEKANALDRISFDLSLARGLDYYTGVIYEAVFKGVTQVGSIAAGGRYDK 344
Query: 270 LSKIY-GKEYPATGGAVYLERLLDILPKNVEKKDYFV 305
L ++ K+ PA G + +ER+ I+ + ++K+ +
Sbjct: 345 LVGMFSNKQVPAVGVSFGIERVFAIMEQQEKEKNQVI 381
sp|Q9ZDL9|SYH_RICPR HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|3860869|emb|CAA14769| (AJ235271) HISTIDYL-TRNA
SYNTHETASE (hisS) [Rickettsia prowazekii]
Length = 416
Score = 62.8 bits (150), Expect = 4e-09
Identities = 80/327 (24%), Positives = 136/327 (41%), Gaps = 31/327 (9%)
Query: 23 IFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGS----KEAITFKDSYTKESFGLRLD 78
I KA + +GY ++ E + + GE S KE +F D + + LR +
Sbjct: 28 IINKARDVGALYGYKQMSTPILEYTKVFNRSMGESSDVMSKEIYSFVDK-SNNAVALRPE 86
Query: 79 FTTQVVRT-ISHLRNVKLPERVYYFGKVFSLDR----RGFEKLQTGVELIGEKSILADFE 133
FT+ ++R+ IS+ KLP + + G VF DR R + Q E +G K + D E
Sbjct: 87 FTSGIIRSFISNGLQHKLPLKFFSTGPVFRYDRPQAGRQRQFHQLNYEYLGAKGAITDAE 146
Query: 134 VIQVLTEFLKSLGLK-DLKVILSHAG-----------IVRKVADEREELLRAFSERNLEA 181
+++ + LK+L ++ D + L+ G +V + D +++L R +
Sbjct: 147 TVKLAVDILKALEIEEDTTLELNSLGCHESRIVYQQKLVEYLNDFKDQLSGESRLRLNKN 206
Query: 182 LKEVLGNDIKFFVEVSKNPEVLNFXXXXXXXXXXXXXXXXXXXXXXFGIDYAYDLGEVRN 241
+L + + ++ + +L+ GI Y+ + VR
Sbjct: 207 PMRILDSKSEIDQKIIAHAPILS---EYHTNESKKYFDELQKYLDILGIKYSVNPRLVRG 263
Query: 242 FPYYTGVIFEIYELKSGK--ALAGGGRYDNLSKIYGK-EYPATGGAVYLERLLDILPKNV 298
YY +FE K G + GGRYD LS+I G + A G A +ER+ + +
Sbjct: 264 LDYYCHTVFEFTTKKLGSQSTILAGGRYDMLSRIMGNYDVHAIGFAAGIERIALMREYKI 323
Query: 299 EK-KDYFVIDKTKKRL--GLKLADVLR 322
K FV+ K + L + D LR
Sbjct: 324 SVIKPVFVLPIGKNNICYALDIVDKLR 350
sp|Q50641|SYH_MYCTU HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|1478232|emb|CAB01263| (Z77724) hisS [Mycobacterium
tuberculosis]
Length = 423
Score = 62.1 bits (148), Expect = 8e-09
Identities = 95/364 (26%), Positives = 143/364 (39%), Gaps = 41/364 (11%)
Query: 35 GYDYINLSHFEPYEFQELTFGEG----SKEAITFKDSYTKESFGLRLDFTTQVVRTISH- 89
GY +I L FE GE SKE TF D + S LR + T VVR +
Sbjct: 39 GYSHIELPIFEDTALFARGVGESTDVVSKEMYTFADRGDR-SVTLRPEGTAGVVRAVIEH 97
Query: 90 -LRNVKLPERVYYFGKVFSLDR----RGFEKLQTGVELIGEKSILADFEVIQVLTEFLKS 144
L LP ++ Y G F +R R + Q GVE IG D EVI + +S
Sbjct: 98 GLDRGALPVKLCYAGPFFRYERPQAGRYRQLQQVGVEAIGVDDPALDAEVIAIADAGFRS 157
Query: 145 LGLKDLKVILSHAGIVRKVADEREELLRAF---SERNLEALKEVLGNDIKFFVEVSKNPE 201
LGL ++ ++ G + ELL+ F + + + + N ++ V K PE
Sbjct: 158 LGLDGFRLEITSLG-DESCRPQYRELLQEFLFGLDLDEDTRRRAGINPLR--VLDDKRPE 214
Query: 202 VLNFXXXX------XXXXXXXXXXXXXXXXXXFGIDYAYDLGEVRNFPYYTGVIFEIYE- 254
+ G+ Y + VR YYT FE
Sbjct: 215 LRAMTASAPVLLDHLSDVAKQHFDTVLAHLDALGVPYVINPRMVRGLDYYTKTAFEFVHD 274
Query: 255 -LKSGKALAGGGRYDNL-SKIYGKEYPATGGAVYLERLLDILPKNVEKK--------DYF 304
L + + GGGRYD L ++ G++ G + ++R +L E K D F
Sbjct: 275 GLGAQSGIGGGGRYDGLMHQLGGQDLSGIGFGLGVDR--TVLALRAEGKTAGDSARCDVF 332
Query: 305 VI---DKTKKRLGLKLADVLREKGKKVGMEIVKERGLEHSLIYAFDKGFKEVLLVEDEIV 361
+ + K RL + LA LR G +V + +RGL+ ++ A G + L+ D +
Sbjct: 333 GVPLGEAAKLRLAV-LAGRLRAAGVRVDL-AYGDRGLKGAMRAAARSGARVALVAGDRDI 390
Query: 362 KVYT 365
+ T
Sbjct: 391 EAGT 394
gi|4103154 (AF020717) histidyl-tRNA synthetase [Triticum aestivum]
Length = 432
Score = 60.5 bits (144), Expect = 2e-08
Identities = 76/337 (22%), Positives = 132/337 (38%), Gaps = 42/337 (12%)
Query: 8 GERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGSKEAITFKDS 67
G R + E+ + + F +F+ G ++ FE E +GE SK D
Sbjct: 1 GTRDFGREQMAIRERAFSIITSVFKMHGGVALDTPVFELRETLMGKYGEDSKLVYDLADQ 60
Query: 68 YTKESFGLRLDFTTQVVRTISHLRNVKLPERVYYFGKVFSLDR----RGFEKLQTGVELI 123
E LR D T R ++ + N+ +R Y KV+ D R E Q ++
Sbjct: 61 -GGELCSLRYDLTVPFARYVA-MNNISAIKR-YQIAKVYRRDNPSKGRYREFYQCDFDIA 117
Query: 124 GE-KSILADFEVIQVLTEFLKSLGLKDLKVILSHAGIV--------------RKVADERE 168
G + + DFEV++VLTE L L + ++ L+H ++ R V +
Sbjct: 118 GVYEPMEPDFEVVKVLTELLDKLDIGVYEIKLNHRKLLDGMLEICGVPAEKFRTVCSSID 177
Query: 169 ELLRAFSER-----------------NLEALKEVLGNDIKFFVEVSKNPEVLNFXXXXXX 211
+L + E N+ +L + G ++ +E+ K
Sbjct: 178 KLDKLTFEEVKKELVEEKGVSNETAENIGSLVKTKGPPLEVLLELRKEGSKFMENEGSVV 237
Query: 212 XXXXXXXXXXXXXXXXFGIDYAYDLGEVRNFPYYTGVIFE--IYELKSGKALAGGGRYDN 269
++DL R YYTGVI+E + ++A GGRYD
Sbjct: 238 ALNELEILFKALEKANALDRISFDLSLARGLDYYTGVIYEAVFKGVTQVGSIAAGGRYDK 297
Query: 270 LSKIY-GKEYPATGGAVYLERLLDILPKNVEKKDYFV 305
L ++ K+ PA G ++ +ER+ I+ + ++K+ +
Sbjct: 298 LVGMFSNKQVPAVGVSLGIERVFAIMEQQEKEKNQVI 334
sp|P43823|SYH_HAEIN HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|1074203|pir||I64063 histidine--tRNA ligase (EC
6.1.1.21) - Haemophilus influenzae (strain Rd KW20)
>gi|1573338 (U32721) histidyl-tRNA synthetase (hisS)
[Haemophilus influenzae Rd]
Length = 423
Score = 59.7 bits (142), Expect = 4e-08
Identities = 93/363 (25%), Positives = 147/363 (39%), Gaps = 34/363 (9%)
Query: 16 ESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEG----SKEAITFKDSYTKE 71
ES L + I + + ++GY + + E GE SKE TF D+ E
Sbjct: 18 ESPLWQWIEAQVRNVLNSYGYSEVRMPIVESTPLFARAIGEVTDVVSKEMYTFWDN--DE 75
Query: 72 SFGLRLDFTTQVVRT-ISHLRNVKLPERVYYFGKVFSLDR----RGFEKLQTGVELIGEK 126
LR + T VR I H +R++Y G +F +R R + Q GVE+ G
Sbjct: 76 QLTLRPEGTAGCVRAAIEHGWIYNNEQRLWYIGPMFRHERPQKGRYRQFHQVGVEVFGIA 135
Query: 127 SILADFEVIQVLTEFLKSLGL-KDLKVILSHAGIVRKVADEREELLRAFSERNLEALKE- 184
+ D E+I + K+LG+ + + + L+ G + A+ R L+ F E + + + +
Sbjct: 136 NPEIDAELIMLTYRLWKALGIDQHVTLQLNSIGSLEARANYRSALV-GFLENHQDLMSDE 194
Query: 185 ----VLGNDIKFFVEVSKNPEVLNFXXXXXXXXXXXXXXXXX------XXXXXFGIDYAY 234
++ N ++ +KNPE+ GI Y
Sbjct: 195 EKERLVRNPLRILD--TKNPELQKVLDNAPKLLDYLDDESRTHFEQLCSLLDAVGIQYEI 252
Query: 235 DLGEVRNFPYYTGVIFE--IYELKSGKALAGGGRYDNL-SKIYGKEYPATGGAVYLERLL 291
+ VR YY +FE L + + GGGRYD L ++ G P+ G A+ LERL+
Sbjct: 253 NPKLVRGLDYYNKTVFEWVTSALGAQGTVCGGGRYDGLVEQLGGHATPSIGFAMGLERLV 312
Query: 292 DILPK---NVEKKDYFVIDKTKKRLGLKLADVLREKGKKVGMEIVKERGLEHSLIYAFDK 348
++ + NV K I + G LA E +KV E+ + H F K
Sbjct: 313 LLVQEVNPNVPAKSAVDIYVVYQGEGATLAAF--ELAEKVRSELPHLNTMLHCSSGNFKK 370
Query: 349 GFK 351
FK
Sbjct: 371 QFK 373
gb|AAF11015.1|AE001989_3 (AE001989) histidyl-tRNA synthetase, putative [Deinococcus
radiodurans]
Length = 406
Score = 58.6 bits (139), Expect = 9e-08
Identities = 62/257 (24%), Positives = 101/257 (39%), Gaps = 30/257 (11%)
Query: 75 LRLDFTTQVVRTI-SHLRNVKLPERVYYFGKVFSLDR-----RGFEKLQTGVELIGEKSI 128
LR ++TT + R + +H + P R+ Y G+++ + R E Q G ELIG +
Sbjct: 93 LRSEYTTALGRLVGTHFPSGPFPLRLQYGGRLWLRTQTSELGRLREFNQVGAELIGVTGV 152
Query: 129 LADFEVIQVLTEFLKSLGLKD---------LKVILSHAGIVRKVADEREELLRAFSERNL 179
AD E++ + L G++ + L+ AG+ V + + S +L
Sbjct: 153 QADAELLALAHAALGQAGVQAQLEVGFPGFVDAALTDAGLPGPVRAALHDAIDRKSGADL 212
Query: 180 EALKEVLG------NDIKFFVEVSKNPEVLN----FXXXXXXXXXXXXXXXXXXXXXXFG 229
+ L G + E+ PEVL G
Sbjct: 213 DLLARQHGVSPEVTRTLHSLTELYGGPEVLTEARVLARGVRAEQAVEHLSAVHAAAQEAG 272
Query: 230 IDYAYDLGEVRNFPYYTGVIFEIYELKSGKALAGGGRYDNLSKIYGKEYPATGGAVYLER 289
++ +DLG R + YYTG+ F Y + + GGGRY + P G A+ LER
Sbjct: 273 VELLFDLGVSRRYGYYTGLTFRAYVDGINQPVLGGGRYALPGGL-----PGAGFAIGLER 327
Query: 290 LLDILPKNVEKKDYFVI 306
L ++P V + V+
Sbjct: 328 LAAVMPAGVPSEPETVL 344
emb|CAA19087.1| (AL023591) histidyl-tRNA synthetase [Mycobacterium leprae]
Length = 427
Score = 57.0 bits (135), Expect = 3e-07
Identities = 91/357 (25%), Positives = 134/357 (37%), Gaps = 33/357 (9%)
Query: 35 GYDYINLSHFEPYEFQELTFGEGS----KEAITFKDSYTKESFGLRLDFTTQVVRTISH- 89
GY I L FE GE + KE TF D + S LR + T VVR +
Sbjct: 42 GYGDIELPVFEDTALFARGVGESTDVVAKEMYTFADRGDR-SVTLRPEGTAGVVRAVIEH 100
Query: 90 -LRNVKLPERVYYFGKVFSLDR----RGFEKLQTGVELIGEKSILADFEVIQVLTEFLKS 144
L LP ++ Y G F +R R + Q GVE IG D EVI + +S
Sbjct: 101 GLDRGALPVKLCYAGPFFRYERPQAGRCRQLQQVGVEAIGVDDPALDAEVITIADAGFRS 160
Query: 145 LGLKDLKVILSHAGIVRKVADEREELLRAFSERNLEALKEVLGNDIKFFVEVSKNPEVLN 204
LGL ++ ++ G R+ L + +L+ V K P+V
Sbjct: 161 LGLDGFQLEITSLGDGTCRPQYRKLLQEFLLQLDLDEDTRRRAELNPLRVLDDKRPQVQA 220
Query: 205 FXXXX------XXXXXXXXXXXXXXXXXXFGIDYAYDLGEVRNFPYYTGVIFEIYE--LK 256
+ Y + VR YYT FE L
Sbjct: 221 MTAAAPVLLDHLSDGAKQHFDTVLAHLDALRVPYVINPRMVRGLDYYTKTTFEFVHPGLG 280
Query: 257 SGKALAGGGRYDNLSK-IYGKEYPATGGAVYLERLLDILPKNVEKK--------DYFVID 307
+ + GGGRYD L + + G++ G + ++R L L + E K D F +
Sbjct: 281 AQSGIGGGGRYDGLMRQLGGQDLSGIGFGLGVDRTL--LALHAEGKTVGETTRCDVFGVS 338
Query: 308 --KTKKRLGLKLADVLREKGKKVGMEIVKERGLEHSLIYAFDKGFKEVLLVEDEIVK 362
+ K LA LR G +V + I +RG+ ++ A G + L+V+D +K
Sbjct: 339 LGEAAKLKVAMLAGQLRAAGVRVDL-IYGDRGIRGAMRAAGRSGARIALIVDDCAIK 394
sp|P46696|SYH_MYCLE HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|2145854|pir||S72739 histidine--tRNA ligase (EC
6.1.1.21) syH - Mycobacterium leprae >gi|466828 (U00011)
hisS; syH; histidyl-tRNA synthetase; B1177_C3_248
[Mycobacterium leprae]
Length = 427
Score = 57.0 bits (135), Expect = 3e-07
Identities = 91/357 (25%), Positives = 134/357 (37%), Gaps = 33/357 (9%)
Query: 35 GYDYINLSHFEPYEFQELTFGEGS----KEAITFKDSYTKESFGLRLDFTTQVVRTISH- 89
GY I L FE GE + KE TF D + S LR + T VVR +
Sbjct: 42 GYGDIELPVFEDTALFARGVGESTDVVAKEMYTFADRGDR-SVTLRPEGTAGVVRAVIEH 100
Query: 90 -LRNVKLPERVYYFGKVFSLDR----RGFEKLQTGVELIGEKSILADFEVIQVLTEFLKS 144
L LP ++ Y G F +R R + Q GVE IG D EVI + +S
Sbjct: 101 GLDRGALPVKLCYAGPFFRYERPQAGRCRQLQQVGVEAIGVDDPALDAEVITIADAGFRS 160
Query: 145 LGLKDLKVILSHAGIVRKVADEREELLRAFSERNLEALKEVLGNDIKFFVEVSKNPEVLN 204
LGL ++ ++ G R+ L + +L+ V K P+V
Sbjct: 161 LGLDGFQLEITSLGDGTCRPQYRKLLQEFLLQLDLDEDTRRRAELNPLRVLDDKRPQVQA 220
Query: 205 FXXXX------XXXXXXXXXXXXXXXXXXFGIDYAYDLGEVRNFPYYTGVIFEIYE--LK 256
+ Y + VR YYT FE L
Sbjct: 221 MTAAAPVLLDHLSDGAKQHFDTVLAHLDALRVPYVINPRMVRGLDYYTKTTFEFVHPGLG 280
Query: 257 SGKALAGGGRYDNLSK-IYGKEYPATGGAVYLERLLDILPKNVEKK--------DYFVID 307
+ + GGGRYD L + + G++ G + ++R L L + E K D F +
Sbjct: 281 AQSGIGGGGRYDGLMRQLGGQDLSGIGFGLGVDRTL--LALHAEGKTVGETTRCDVFGVS 338
Query: 308 --KTKKRLGLKLADVLREKGKKVGMEIVKERGLEHSLIYAFDKGFKEVLLVEDEIVK 362
+ K LA LR G +V + I +RG+ ++ A G + L+V+D +K
Sbjct: 339 LGEAAKLKVAMLAGQLRAAGVRVDL-IYGDRGIRGAMRAAGRSGARIALIVDDCAIK 394
emb|CAA17892.1| (AL022103) histidyl-trna synthetase, mitochondrial precursor
[Schizosaccharomyces pombe]
Length = 538
Score = 56.6 bits (134), Expect = 3e-07
Identities = 77/351 (21%), Positives = 133/351 (36%), Gaps = 69/351 (19%)
Query: 19 LVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGSKEAITFKDSYTKESFGLRLD 78
L + IF +IF+ G ++ FE E +GE SK KD E LR D
Sbjct: 79 LREHIFSTVTEIFKRHGGVTLDTPVFELKEILSGKYGEDSKLIYDLKDQ-GGELCSLRYD 137
Query: 79 FTTQVVRTISHLRNVKLPERVYYFGKVFSLDRRGFEK------LQTGVELIGEKS-ILAD 131
T R ++ V +R ++ KV+ D+ K Q ++ G ++ D
Sbjct: 138 LTVPFARWLAMNPKVTSIKR-FHIAKVYRRDQPAMTKGRMREFYQCDFDIAGNYDPMIPD 196
Query: 132 FEVIQVLTEFLKSLGLKDLKVILSHAGI-----------------VRKVADEREELLRAF 174
E ++VL E L +L + + + L+H I + D+ ++L
Sbjct: 197 SEALKVLVEALDALEVGNYTIKLNHRKILDGIFTVCGVPDDKIRTISSAVDKLDKLPWED 256
Query: 175 SERNL---EALKEVLGNDIKFFVEVSKNPEVLN-------FXXXXXXXXXXXXXXXXXXX 224
R + + LKE + + IK +V + + +L+
Sbjct: 257 VRREMVVEKGLKEEVADKIKEYVLLKGDRSLLDKLEADSLLSSNSSAVAAFNDMRLLFDY 316
Query: 225 XXXFGI--DYAYDLGEVRNFPYYTGVIFE-IYELKSGK---------------------- 259
FG+ +++D+ R YYTG+I+E + E + K
Sbjct: 317 LEAFGVLDRFSFDMSLARGLDYYTGIIYEAVTEASAPKIKSSAEKKKSADPEADRSNDDS 376
Query: 260 ----ALAGGGRYDNLSKIY----GKEYPATGGAVYLERLLDILPKNVEKKD 302
++A GGRYDNL ++ + P G ++ LER+ IL + +D
Sbjct: 377 IGVGSIAAGGRYDNLVGMFAAKKNAKIPCVGISLGLERIFSILRSKIPDED 427
sp|P49590|SYHH_HUMAN HISTIDYL-TRNA SYNTHETASE HOMOLOG (HISTIDINE--TRNA LIGASE HOMOLOG)
(HISRS) >gi|899109 (U18937) histidyl-tRNA synthetase
homologue [Homo sapiens]
Length = 506
Score = 55.8 bits (132), Expect = 6e-07
Identities = 96/416 (23%), Positives = 158/416 (37%), Gaps = 65/416 (15%)
Query: 8 GERFYSLEESNLVKEIFKKAVKIFEAFGYDYINLSHFEPYEFQELTFGEGSKEAITFKDS 67
G R S + + ++I + F+ G ++ FE E +GE S KD
Sbjct: 62 GTRDLSPQHMVVREKILDLVISCFKRHGAKGMDTPAFELKETLTEKYGEDSGLMYDLKDQ 121
Query: 68 YTKESFGLRLDFTTQVVRTISHLRNVKLPERVYYFGKVFSLDR------RGFEKLQTGVE 121
E LR D T R ++ + VK +R Y+ GKV+ + R E Q +
Sbjct: 122 -GGELLSLRYDLTVPFARYLA-MNKVKKMKR-YHVGKVWRRESPTIVQGRYREFCQCDFD 178
Query: 122 LIGE-KSILADFEVIQVLTEFLKSLGLKDLKVILSHAGIVR---KVADEREELLRAFSER 177
+ G+ ++ D E ++++ E L L L D + ++ IV V E RA
Sbjct: 179 IAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESKFRAICS- 237
Query: 178 NLEALKEVLGNDIKFFVEVSKN--PEVLN-----------------------FXXXXXXX 212
+++ L ++ D++ + V K PEV +
Sbjct: 238 SIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQAL 297
Query: 213 XXXXXXXXXXXXXXXFGI--DYAYDLGEVRNFPYYTGVIFEIYELKSGK----------A 260
FGI ++DL R YYTGVI+E L++ +
Sbjct: 298 EGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGS 357
Query: 261 LAGGGRYDNLSKIY---GKEYPATGGAVYLERLLDILPKNVEKK---------DYFVIDK 308
+A GGRYD L ++ G + P G ++ +ER+ I+ + ++ K FV
Sbjct: 358 VAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATP 417
Query: 309 TKKRL--GLKLADVLREKGKKVGMEIVKERGLEHSLIYAFDKGFKEVLLVEDEIVK 362
K L LKL L + G K M L L Y G V+++ ++ +K
Sbjct: 418 QKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELK 473
sp|P47281|SYH_MYCGE HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|1361544|pir||H64203 histidine--tRNA ligase (EC
6.1.1.21) - Mycoplasma genitalium (SGC3) >gi|3844645
(U39683) histidyl-tRNA synthetase [Mycoplasma
genitalium]
Length = 414
Score = 51.5 bits (121), Expect = 1e-05
Identities = 64/286 (22%), Positives = 116/286 (40%), Gaps = 29/286 (10%)
Query: 59 KEAITFKDSYTKESFGLRLDFTTQVVRTISH---LRNVKLPERVYYFGKVFSLDR----R 111
KE F D +E LR + TT +VR ++ P +++ G ++ +R R
Sbjct: 63 KELYQFFDKSQRE-LALRPEATTPIVRLACENKLMQEANFPLKLFCIGSMYRYERPQNNR 121
Query: 112 GFEKLQTGVELIGEKSILADFEVIQVLTEFLKSLGLKDLKVILSHAGIVRKVADEREEL- 170
E Q E+ G ++ + + L++LG+ + +++ ++ + L
Sbjct: 122 FREHWQFSCEVFGFSNLFIFLDTLLFANSLLEALGITGYVLKINNLANFETLSKWNKALK 181
Query: 171 ---------LRAFSERNLEALK-EVLGNDIKFFVEVSKN-PEVLNFXXXXXXXXXXXXXX 219
L S++ LE +L + I KN P++ +F
Sbjct: 182 DYLTPYKLELTELSQKRLEKNPLRILDDKIDQKKSFVKNAPKITDFLDASAKQDSELLKT 241
Query: 220 XXXXXXXXFGIDYAYDLGEVRNFPYYTGVIFEIYELKSGKALAGGGRYDNL-SKIYGKEY 278
I + + VR YYTG +FE +K+ + GG YDNL ++
Sbjct: 242 QLKKH----NISFEWTDNLVRGLDYYTGFVFEY--VKNQDTILAGGVYDNLVEELSSNPT 295
Query: 279 PATGGAVYLERLLDILPKNVEKKDYFVIDKTKKRLGLKLADVLREK 324
PA G A +ERL++ L ++KK + + K K+ L + L + E+
Sbjct: 296 PALGFACGIERLINCL--EIDKKAFILNTKPKQMLVICLFEEALEE 339
sp|P46220|SYH_SULSH PUTATIVE HISTIDYL-TRNA SYNTHETASE (HISTIDINE--TRNA LIGASE) (HISRS)
>gi|557202 (L36863) homologous to N-terminal part of
Histidyl-tRNA synthetase (P30053); ORF1 [Sulfolobus
shibatae]
Length = 138
Score = 48.8 bits (114), Expect = 7e-05
Identities = 41/124 (33%), Positives = 63/124 (50%), Gaps = 12/124 (9%)
Query: 15 EESNLVKEIFKK-AVKIFEAFGYDYINLSHFEPYEFQELTFGEGSKEAI-TFKDSYTKES 72
E+ ++ IF+ A+K +GY I E ++ L GE +E + FKD +E
Sbjct: 19 EKIRYLESIFRDLAIK----YGYSEIITPVVEEFKLFALKGGEELRETMYVFKDKADRE- 73
Query: 73 FGLRLDFTTQVVRT-ISHLRNVKLPERVYYFGKVFSLDRRGF----EKLQTGVELIGEKS 127
LR + T V R I +L++ P R++YFG V+ D + E Q G+E+IG+ S
Sbjct: 74 LSLRPEITPGVARAYIQNLQSSPKPIRLFYFGTVYRYDEPQYGRYREFRQAGIEMIGDSS 133
Query: 128 ILAD 131
IL D
Sbjct: 134 ILDD 137
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
Posted date: Jan 12, 2000 2:26 PM
Number of letters in database: 136,724,657
Number of sequences in database: 444,417
Lambda K H
0.320 0.142 0.392
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 95288844
Number of Sequences: 444417
Number of extensions: 3996001
Number of successful extensions: 10083
Number of sequences better than 10.0: 70
Number of HSP's better than 10.0 without gapping: 25
Number of HSP's successfully gapped in prelim test: 45
Number of HSP's that attempted gapping in prelim test: 9932
Number of HSP's gapped (non-prelim): 120
length of query: 383
length of database: 136,724,657
effective HSP length: 57
effective length of query: 326
effective length of database: 111,392,888
effective search space: 36314081488
effective search space used: 36314081488
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 71 (32.1 bits)
