Score E
Sequences producing significant alignments: (bits) Value
gi|2983621 (AE000726) putative protein [Aquifex aeolicus] 518 e-146
gi|2983654 (AE000728) putative protein [Aquifex aeolicus] 265 3e-70
gi|2983965 (AE000748) lipoate-protein ligase A [Aquifex aeo... 116 2e-25
ref|NP_006283.1|| tumor susceptibility gene 101 >gi|3184258... 33 2.9
emb|CAA67419| (X98917) formylmethanofuran dehydrogenase Fwu... 32 3.8
emb|CAB06230| (Z83864) hypothetical protein Rv3860 [Mycobac... 32 3.8
sp|Q92345|DCP2_SCHPO PROBABLE PYRUVATE DECARBOXYLASE C1F8.0... 32 6.5
dbj|BAA13925| (D89264) similar to Neurospore crassa pyruva... 31 8.5
gi|2983621 (AE000726) putative protein [Aquifex aeolicus]
Length = 244
Score = 518 bits (1319), Expect = e-146
Identities = 244/244 (100%), Positives = 244/244 (100%)
Query: 1 MWRLYMAKKNPIQPGPEGYIPTPAAFQGCDLPPPGKALLYGEIVDEEVAMREAAKALLTR 60
MWRLYMAKKNPIQPGPEGYIPTPAAFQGCDLPPPGKALLYGEIVDEEVAMREAAKALLTR
Sbjct: 1 MWRLYMAKKNPIQPGPEGYIPTPAAFQGCDLPPPGKALLYGEIVDEEVAMREAAKALLTR 60
Query: 61 RNPTIFPGPLVLWGWNADAMEKAKAVLELAMEIPNCRIIPMPDYRPKYPKIDPEAEINPN 120
RNPTIFPGPLVLWGWNADAMEKAKAVLELAMEIPNCRIIPMPDYRPKYPKIDPEAEINPN
Sbjct: 61 RNPTIFPGPLVLWGWNADAMEKAKAVLELAMEIPNCRIIPMPDYRPKYPKIDPEAEINPN 120
Query: 121 HPNLTILHNKIEACIFVGVHCHYANLSLRMIRAGTNCFTIALCAEMGHEDAMVSLRDVHA 180
HPNLTILHNKIEACIFVGVHCHYANLSLRMIRAGTNCFTIALCAEMGHEDAMVSLRDVHA
Sbjct: 121 HPNLTILHNKIEACIFVGVHCHYANLSLRMIRAGTNCFTIALCAEMGHEDAMVSLRDVHA 180
Query: 181 DEIRKFRDVVVEVRKELGIEWEPKLPPENPSLPKEDWVKISPLDFGEYAYLLIPRRGEIV 240
DEIRKFRDVVVEVRKELGIEWEPKLPPENPSLPKEDWVKISPLDFGEYAYLLIPRRGEIV
Sbjct: 181 DEIRKFRDVVVEVRKELGIEWEPKLPPENPSLPKEDWVKISPLDFGEYAYLLIPRRGEIV 240
Query: 241 EESE 244
EESE
Sbjct: 241 EESE 244
gi|2983654 (AE000728) putative protein [Aquifex aeolicus]
Length = 189
Score = 265 bits (670), Expect = 3e-70
Identities = 124/185 (67%), Positives = 151/185 (81%), Gaps = 2/185 (1%)
Query: 15 GPEGYIPTPAAF-QGCDLPPPGKALLYGEIVDEEVAMREAAKALLTRRNPTIFPGPLVLW 73
GP GY P P A +G PPPGKAL++ EIVDEE+AMREAAKA+LTR NPTIFPGP VL+
Sbjct: 5 GPAGYSPYPVAVTEGVLTPPPGKALMFNEIVDEEIAMREAAKAMLTRDNPTIFPGPQVLY 64
Query: 74 GWNADAMEKAKAVLELAMEIPNCRIIPMPDYRPKYPKIDPEAEINPNHPNLTILHNKIEA 133
WN +A EKAK V ++A E+ +IIPM DYRPKYPKIDPE EINPNHPNLTI HNKI+A
Sbjct: 65 AWNEEAKEKAKLVRKMA-EVLGAKIIPMYDYRPKYPKIDPEKEINPNHPNLTIWHNKIKA 123
Query: 134 CIFVGVHCHYANLSLRMIRAGTNCFTIALCAEMGHEDAMVSLRDVHADEIRKFRDVVVEV 193
CIF+GVHCHYAN++L++IRA T+CFTIA+C+ GHEDAM++LRD H +++ KF + EV
Sbjct: 124 CIFIGVHCHYANVALKIIRAETDCFTIAMCSMAGHEDAMITLRDQHVEDLEKFIKIAEEV 183
Query: 194 RKELG 198
+KELG
Sbjct: 184 KKELG 188
gi|2983965 (AE000748) lipoate-protein ligase A [Aquifex aeolicus]
Length = 788
Score = 116 bits (287), Expect = 2e-25
Identities = 52/149 (34%), Positives = 91/149 (60%)
Query: 41 GEIVDEEVAMREAAKALLTRRNPTIFPGPLVLWGWNADAMEKAKAVLELAMEIPNCRIIP 100
G+ V E A++ A + L + PT+ GPLVLW WN +A +K + + L + PN I
Sbjct: 639 GQKVSFEEALQVAVELLSKAKRPTLIVGPLVLWAWNEEAKKKGEIIKRLKEKYPNLNIHL 698
Query: 101 MPDYRPKYPKIDPEAEINPNHPNLTILHNKIEACIFVGVHCHYANLSLRMIRAGTNCFTI 160
+PDY+PK K+D E++P +P+L++L+ + + + +GVHC+ + ++RM++ T+ +
Sbjct: 699 LPDYKPKNKKVDFSREVDPPNPHLSVLYGEHDLTLMIGVHCYRTDFTIRMLKKYTDTKIV 758
Query: 161 ALCAEMGHEDAMVSLRDVHADEIRKFRDV 189
LC GH DA VSL V++ ++ F ++
Sbjct: 759 TLCTLYGHPDADVSLAGVNSKKLEMFYEM 787
ref|NP_006283.1|| tumor susceptibility gene 101 >gi|3184258 (U82130) tumor
susceptibility protein [Homo sapiens]
Length = 390
Score = 32.8 bits (73), Expect = 2.9
Identities = 12/24 (50%), Positives = 14/24 (58%)
Query: 12 IQPGPEGYIPTPAAFQGCDLPPPG 35
I P P GY P P+ + GC PP G
Sbjct: 171 ISPYPSGYPPNPSGYPGCPYPPGG 194
emb|CAA67419| (X98917) formylmethanofuran dehydrogenase FwuB [Methanopyrus
kandleri]
Length = 433
Score = 32.5 bits (72), Expect = 3.8
Identities = 37/154 (24%), Positives = 60/154 (38%), Gaps = 18/154 (11%)
Query: 44 VDEEVAMREAAKALLTRRNPTIFPGPLVLWGWNADAMEKAKAVLELAMEIPNCRIIPMPD 103
+D E A E AK L+ + P VL+GW+A +E + +ELA + II
Sbjct: 67 IDYEDAAEETAKMLVEAKLP-------VLYGWSATLVEAQEKGVELAELVGG--IIDNTA 117
Query: 104 YRPKYPKIDPEAEINPNHPNLTILHNKIEACIFVG---VHCHYANLSLRMIRAGTNCFTI 160
P + ++ L + N+ + I+ G +H H ++S FT
Sbjct: 118 SVXHGPSVLGLQDVGVPSCTLGEVKNRADTVIYWGSNPMHAHPRHMS------RYTAFTR 171
Query: 161 ALCAEMGHEDAMVSLRDVHADEIRKFRDVVVEVR 194
G ED + + D + DV + VR
Sbjct: 172 GFFRPKGREDRTIIVVDPRKTATARLADVYIRVR 205
emb|CAB06230| (Z83864) hypothetical protein Rv3860 [Mycobacterium tuberculosis]
Length = 390
Score = 32.5 bits (72), Expect = 3.8
Identities = 14/27 (51%), Positives = 16/27 (58%), Gaps = 1/27 (3%)
Query: 11 PIQPG-PEGYIPTPAAFQGCDLPPPGK 36
P QPG P GY P P + C PPPG+
Sbjct: 14 PHQPGTPRGYSPRPPSGDRCPAPPPGR 40
sp|Q92345|DCP2_SCHPO PROBABLE PYRUVATE DECARBOXYLASE C1F8.07C
>gi|1638854|emb|CAB03601.1| (Z81312) pyruvate
decarboxylase [Schizosaccharomyces pombe]
Length = 594
Score = 31.7 bits (70), Expect = 6.5
Identities = 27/85 (31%), Positives = 41/85 (47%), Gaps = 8/85 (9%)
Query: 20 IPTPAAFQGCDLPPPGKALLYGEIVDEEVAMR--EAAKALLTRRNPTIFPGPLVLWGWNA 77
IPT A Q C +P P A++ EI D+E + + A L++++ P++L G
Sbjct: 172 IPTNMANQPCPVPGPISAVISPEISDKESLEKATDIAAELISKKEK-----PILLAGPKL 226
Query: 78 DAMEKAKAVLELAMEIPNCRIIPMP 102
A A ++LA E NC MP
Sbjct: 227 RAAGAESAFVKLA-EALNCAAFIMP 250
dbj|BAA13925| (D89264) similar to Neurospore crassa pyruvate decarboxylase,
SWISS-PROT Accession Number P33287 [Schizosaccharomyces
pombe]
Length = 605
Score = 31.3 bits (69), Expect = 8.5
Identities = 27/85 (31%), Positives = 41/85 (47%), Gaps = 8/85 (9%)
Query: 20 IPTPAAFQGCDLPPPGKALLYGEIVDEEVAMR--EAAKALLTRRNPTIFPGPLVLWGWNA 77
IPT A Q C +P P A++ EI D+E + + A L++++ P++L G
Sbjct: 172 IPTNMANQPCAVPGPISAVISPEISDKESLEKATDIAAELISKKEK-----PILLAGPKL 226
Query: 78 DAMEKAKAVLELAMEIPNCRIIPMP 102
A A ++LA E NC MP
Sbjct: 227 RAAGAESAFVKLA-EALNCAAFIMP 250
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
Posted date: Jan 12, 2000 2:26 PM
Number of letters in database: 136,724,657
Number of sequences in database: 444,417
Lambda K H
0.320 0.140 0.444
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 75881251
Number of Sequences: 444417
Number of extensions: 3384496
Number of successful extensions: 7599
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 7593
Number of HSP's gapped (non-prelim): 8
length of query: 244
length of database: 136,724,657
effective HSP length: 49
effective length of query: 195
effective length of database: 114,948,224
effective search space: 22414903680
effective search space used: 22414903680
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 69 (31.3 bits)
