Spizellomyces punctatus mtDNA

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We have determined the complete sequence of the 61.3 kbp long mtDNA of the chytridiomycete fungus, Spizellomyces punctatus (Barr), which is organized in three (one big and two small 1.5 kbp) circular chromosomes. One of the two small circles codes for the atp9 gene, whereas the other one carries only a conserved promoter-like element but no identified gene. The set of identified genes is typical for fungal mitochondria, and contain several group I introns (three in cob, two in cox1, one in cox3 and two in nad5. The number of identified tRNA genes (eight) is insufficient to read all codons of the mitochondrial protein genes (as in Harpochytrium and in Rhizophydium), and the tRNAs have be edited in order to conform to a standard structural model (as in Harpochytrium). The protein coding genes contain many in-frame UAG "stop" codons, which are likely translated as leucine. In fact, one of tRNAs has an anticodon that would specifically recognize UAG codons, and this tRNA has a striking similarity to leucine tRNAs from other species (including a long extra arm)

The phylogenetic analysis places Spizellomyces , a member of the Spizellomycetales, into the same major lineage as Rhizophydium (Chytridiales) and Rhizophlyctis rosea, but very distant from the Monoblepharidales. Its closest phylogenetic neighbor is Rhizophlyctis rosea, a species which was previously not well classified, in taxonomic terms.


[ Allomyces | Aspergillus| Harpochytrium | Monoblepharella | Monosiga | Phytophtora | Rhizophydium | Rhizopus | Schizophyllum | Schizosaccharomyces | Spizellomyces ]