The Fungal Mitochondrial Genome Project: evolution of fungal mitochondrial genomes and their gene expression

Bruno Paquin, Marie-Josée Laforest, Lise Forget, Ingeborg Roewer, Zhang Wang, Joyce Longcore and B. Franz Lang*

^*Département de Biochimie, Université de Montréal, CP 6128 succ. Centre-Ville, Montréal, Québec H3C 3J7, Canada

Abstract

The goal of the fungal Mitochondrial Genome Project (FMGP) is to sequence complete mitochondrial genomes for a representative sample of the major fungal lineages ; to analyze the genome structure, gene content, and conserved sequence elements of these sequences; and to study the evolution of gene expression in fungal mitochondria. By using our new sequence data for evolutionary studies, we were able to construct phylogenetic trees that provide further solid evidence that animals and fungi share a common ancestor to the exclusion of chlorophytes and protists. With a database comprising multiple mitochondrial gene sequences, the level of support for our mitochondrial phylogenies is unprecedented, in comparision to trees inferred with nuclear ribosomal RNA sequences. We also found several new molecular features in the mitochondrial genomes of lower fungi, including: (i) tRNA editing, which is the same type as that found in the mitochondria of the amoeboid protozoan, Acanthamoeba castellanii; (ii) two novel types of putative mobile DNA elements, one encoding a site-specific endonuclease that confers mobility on the element, and the other constituting a class of highly compact, structured elements; and (iii) a high number of introns, which provide insights into intron origins and evolution. Here, we present an overview of these results and discuss examples of the diversity of structures found in the fungal mitochondrial genome.