WCG and WCL medium, for many protists

WCL medium seems to give better growth than WCG medium, in most cases.
To one liter of distilled and autoclaved water add: 
                                         WCG            WCL

K2HPO4*        ( 8.71 g/l)              0.25 ml         ----
CaCl2x2H2O     (36.76 g/l)              0.1  ml        1.0 ml
MgSO4x7H2O     (36.97 g/l)              0.25 ml        1.0 ml
NaNO3*         (75.0  g/l)              0.29 ml        1.0 ml
NaHCO3*        (12.6  g/l)              0.05 ml        1.0 ml
H3BO3*         ( 6.0  g/l)              0.25 ml        0.5 ml
KCL*           ( 7.45 g/l)               ----          1.0 ml
NaH2PO4xH2O    ( 5.0     g/l)            ----          1.0 ml
NH4CL*         ( 2.65 g/l)               ----          1.0 ml
Na2EDTA*       (100mM Stock)            0.05 ml         ----

Fe/EDTA/trace metals from F/2 stock*    0.25 ml        1.0 ml
F/2 vitamins*                           0.5  ml        1.0 ml
(see on F/2 page)
In order to obtain high cell yields, buffering with HEPES is strongly recommended,

HEPES buffer: stock of 1M, adjusted to pH 7.5, end concentration in medium should be about 10-20 mM.

[ One level back | OGMP | FMGP | GOBASE | PID | CIAR-PEB | ISEP | CGAT | Comments | WebSearch ]