WCG WCL K2HPO4* ( 8.71 g/l) 0.25 ml ---- CaCl2x2H2O (36.76 g/l) 0.1 ml 1.0 ml MgSO4x7H2O (36.97 g/l) 0.25 ml 1.0 ml NaNO3* (75.0 g/l) 0.29 ml 1.0 ml NaHCO3* (12.6 g/l) 0.05 ml 1.0 ml H3BO3* ( 6.0 g/l) 0.25 ml 0.5 ml KCL* ( 7.45 g/l) ---- 1.0 ml NaH2PO4xH2O ( 5.0 g/l) ---- 1.0 ml NH4CL* ( 2.65 g/l) ---- 1.0 ml Na2EDTA* (100mM Stock) 0.05 ml ---- Fe/EDTA/trace metals from F/2 stock* 0.25 ml 1.0 ml F/2 vitamins* 0.5 ml 1.0 ml (see on F/2 page)
HEPES buffer: stock of 1M, adjusted to pH 7.5, end concentration in medium should be about 10-20 mM.