YBDG Medium for Smittium and Basidiobolus

For one liter medium:
                                 
     brain heart infusion (Difco)          3,7 g
     tryptone                              5   g
     yeast extract                         5   g
     K2HPO4                                0.3 g
     KH2PO4                                0.3 g
     glycerol                              1 %

     (pH comes out to about 6.8)

     For solid media add 1,7 % Agar

     Autoclave 

Add 2% Glucose, 2 mg thiamine and 0.05 mg biotin from 
sterile 50% stock, before use.

For liquid medium use about 200 mL in a 2L Erlenmeyer flasks, inoculate with cells from petri dish, and incubate at room temperature (with vivorous shaking ), for a few days. Growth is very fast and heavy in this medium.

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