Modified Yeast Standard Medium (YD+NB) for Rhizophlyctis rosea


For one liter medium:
                                 
     0,17% Nitrogen Base          1,7 g
     0,5% yeast extract             5 g
     Ajust pH 5,8 with K2HPO4
     For solid media add 1,7% Agar

     Autoclave 

Add 3% Glucose from sterile 50% stock, before use.  


For liquid medium use about 200 mL in a 2L Erlenmeyer flasks, inoculate with cells from petri dish, and incubate at room temperature (without shaking !), for several weeks. To inoculate fresh liquid media, use liquid cultures containing zoospores.

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