Bristol's medium
This medium can be used to grow many non-photosynthetic
fresh water protists, which use bacteria as food source (e.g., Reclinomonas
americana, Cafeteria roenbergensis, Monosiga brevicollis, Ministeria vibrans
etc.). Add freshly grown Enterobacter aerogenes (ATTC 13048), or
any other non-pathogenic food bacteria to the medium described below. Do
not overfeed, daily servings are sufficient. Only at high cell densities,
during the late logarithmic growth phase, one can add an amount of bacteria
that were fully grown on one LB petri dish (over nite), to one liter
of culture. It is preferable to shake cultures at low speed (about 100
rpm), but not all protists will support so.
To 1l distilled and autoclaved water add:
add ml Steril stock solutions
10 ml CaCl2 (1g/400ml)
10 ml MgSO4x7H2O (3g/400ml)
10 ml of Bristol salt mix (3g K2HPO4, 7g KH2PO4,
1g NaCl, 10 g NaNO3 /400ml)