YBDG Medium for Amoebidium (biotin added)
For one liter medium:
brain heart infusion (Difco) 3,7 g
tryptone 5 g
yeast extract 5 g
K2HPO4 0.3 g
KH2PO4 0.3 g
glycerol 1 %
(pH comes out to about 6.8)
For solid media add 1,7 % Agar
Autoclave
Add 2% Glucose, 2 mg thiamine and 0.05 mg biotin from
sterile 50% stock, before use.
Amoebidium is a slow grower, and it is important to start well-grown
starter cultures before going to larger volumes. For liquid medium use
about 200 mL in a 2L Erlenmeyer flasks, inoculate with cells from petri
dish, and incubate at room temperature (slow shaking), for several weeks.